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Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study
- Source :
- Clinical Immunology, Clinical Immunology, Elsevier, 2017, ⟨10.1016/j.clim.2017.09.019⟩, Clinical Immunology, 2017, ⟨10.1016/j.clim.2017.09.019⟩, Clinical Immunology, Elsevier, 2017, 〈10.1016/j.clim.2017.09.019〉, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname
- Publication Year :
- 2017
-
Abstract
- Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture (R), which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMC5 from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies. One sentence summary: A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses. (C) 2017 Published by Elsevier Inc.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Whole blood stimulation
CD3 Complex
medicine.medical_treatment
CD8 Antigens
Point-of-Care Systems
Immunology
Stimulation
Blood Donors
Immunologic Tests
Peripheral blood mononuclear cell
Antibodies
Functional immune responses
03 medical and health sciences
Immune system
medicine
[ SDV.IMM ] Life Sciences [q-bio]/Immunology
Immunology and Allergy
Humans
Whole blood
biology
business.industry
Immunotherapy
Multi-center clinical studies
3. Good health
Vaccination
Immunomonitoring
030104 developmental biology
Gene Expression Regulation
PBMCs
biology.protein
[SDV.IMM]Life Sciences [q-bio]/Immunology
Cytokines
Antibody
business
Biomarkers
Subjects
Details
- ISSN :
- 15217035 and 15216616
- Volume :
- 183
- Database :
- OpenAIRE
- Journal :
- Clinical immunology (Orlando, Fla.)
- Accession number :
- edsair.doi.dedup.....0aebb57017b24ab9f5acc43e61dabc17