High-speed tracking of murine cardiac stem cells by harmonic nanodoublers

Authors :: Ronan Le Dantec
Jean-Pierre Wolf
Yannick Mugnier
Daniel Ciepielewski
Jérôme Extermann
Pernilla Hoffmann
Luigi Bonacina
Thibaud Magouroux
Christelle Kasparian
Marisa Jaconi
Group of Applied Physics [Geneva] (GAP)
University of Geneva [Switzerland]
Laboratoire SYstèmes et Matériaux pour la MEcatronique (SYMME)
Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Source :: Small, Vol. 8, No 17 (2012) pp. 2752-6, Small, Small, Wiley-VCH Verlag, 2012, 8 (17), pp. 2752-2756. ⟨10.1002/smll.201200366⟩
Publication Year :: 2012
Abstract: International audience; Potassium niobate nonlinear nanoparticles are used for the fi rst time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength fl exibility. The potential of this approach is made evident for tissueregeneration studies and applications, by capturing a high-speed movie of ESCderived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.