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Evidence for a Proinflammatory and Proteolytic Environment in Plaques From Endarterectomy Segments of Human Carotid Arteries
- Source :
- Arteriosclerosis, Thrombosis, and Vascular Biology. 24:129-135
- Publication Year :
- 2004
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2004.
-
Abstract
- Objectives Based on previous observations on apolipoprotein(a), apo(a), in human unstable carotid plaques, we explored whether in the inflammatory environment of human atheroma, proteolytic events affect other hepatic and topically generated proteins in relation to the issue of plaque stability. Methods and Results Forty unstable and 24 stable plaques from endarterectomy segments of affected human carotid arteries were extracted with buffered saline (PBS) and then 6 mol/L guanidine-hydrochloride (GdHCl) to identify loosely and tightly bound products, respectively. The extracts were studied before and after ultracentrifugation at d 1.21 g/mL. In the extracts, the concentrations of interleukin (IL)-6, −8, and −18 were significantly higher in the unstable plaques and correlated to those of MMP-2 and MMP-9. By Western blots, both apoB and apo(a) were highly fragmented and mostly present in the d 1.21 bottom that also contained fragments of apoE (10 and 22 kDa), decorin, biglycan, and versican. Fragmentation was higher in the unstable plaques. In baseline plasmas, concentrations of lipids, lipoproteins, and ILs did not differ between patients with unstable and stable plaques. Conclusions In unstable and to a lesser extent in stable plaques, there is a proinflammatory and proteolytic microenvironment with the generation of fragments with potential pathobiological significance that requires investigation.
- Subjects :
- Male
Vasculitis
Apolipoprotein E
Pathology
medicine.medical_specialty
Apolipoprotein B
Decorin
Proinflammatory cytokine
medicine
Humans
Carotid Stenosis
Guanidine
Aged
Endarterectomy, Carotid
Extracellular Matrix Proteins
biology
business.industry
Interleukins
Biglycan
Proteins
Middle Aged
medicine.disease
Molecular biology
Matrix Metalloproteinases
Peptide Fragments
Apolipoproteins
Atheroma
Proteoglycan
biology.protein
Versican
Female
Proteoglycans
Cardiology and Cardiovascular Medicine
business
Biomarkers
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....0af46c2077d16f636ce75593b4e9f5c8
- Full Text :
- https://doi.org/10.1161/01.atv.0000104013.71118.53