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Evidence for a Proinflammatory and Proteolytic Environment in Plaques From Endarterectomy Segments of Human Carotid Arteries

Authors :
Marilena Formato
Celina Edelstein
Paolo Biglioli
Anna Guarino
Cherchi Gm
Angelo M. Scanu
Marco Maggioni
Rita Spirito
Miriam Farina
Source :
Arteriosclerosis, Thrombosis, and Vascular Biology. 24:129-135
Publication Year :
2004
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2004.

Abstract

Objectives Based on previous observations on apolipoprotein(a), apo(a), in human unstable carotid plaques, we explored whether in the inflammatory environment of human atheroma, proteolytic events affect other hepatic and topically generated proteins in relation to the issue of plaque stability. Methods and Results Forty unstable and 24 stable plaques from endarterectomy segments of affected human carotid arteries were extracted with buffered saline (PBS) and then 6 mol/L guanidine-hydrochloride (GdHCl) to identify loosely and tightly bound products, respectively. The extracts were studied before and after ultracentrifugation at d 1.21 g/mL. In the extracts, the concentrations of interleukin (IL)-6, −8, and −18 were significantly higher in the unstable plaques and correlated to those of MMP-2 and MMP-9. By Western blots, both apoB and apo(a) were highly fragmented and mostly present in the d 1.21 bottom that also contained fragments of apoE (10 and 22 kDa), decorin, biglycan, and versican. Fragmentation was higher in the unstable plaques. In baseline plasmas, concentrations of lipids, lipoproteins, and ILs did not differ between patients with unstable and stable plaques. Conclusions In unstable and to a lesser extent in stable plaques, there is a proinflammatory and proteolytic microenvironment with the generation of fragments with potential pathobiological significance that requires investigation.

Details

ISSN :
15244636 and 10795642
Volume :
24
Database :
OpenAIRE
Journal :
Arteriosclerosis, Thrombosis, and Vascular Biology
Accession number :
edsair.doi.dedup.....0af46c2077d16f636ce75593b4e9f5c8
Full Text :
https://doi.org/10.1161/01.atv.0000104013.71118.53