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Echinocystic acid ameliorates hyperhomocysteinemia-induced vascular endothelial cell injury through regulating NF-κB and CYP1A1
- Source :
- Experimental and Therapeutic Medicine
- Publication Year :
- 2017
- Publisher :
- D.A. Spandidos, 2017.
-
Abstract
- The present study investigated the role of echinocystic acid (EA) on the expression of nuclear factor (NF)-κB and cytochrome P450 1A1 (CYP1A1), and aortic morphology, in a rat model of hyperhomocysteinemia (Hhcy). A total of 50 Sprague Dawley rats were randomly divided into five groups as follows: Normal control (NC), model control (MC), vitamin control (VC; folic acid 1 mg/kg + vitamin B2 2 mg/kg + vitamin B12 10u g/kg), EA1 (20 mg/kg EA) and EA2 (40 mg/kg EA). Plasma homocysteine (Hcy) levels were determined via high performance liquid chromatography, and the morphology of the aorta was investigated using hematoxylin and eosin staining. Furthermore, aortic mRNA and protein levels of NF-κB and CYP1A1 were measured using reverse transcription-quantitative polymerase chain reaction analysis and western blotting, respectively. Plasma Hcy levels, and aortic mRNA and protein levels of NF-κB and CYP1A1, were significantly lower in the EA-treated group compared with the MC group (all P
- Subjects :
- 0301 basic medicine
Vitamin
Cancer Research
medicine.medical_specialty
Pathology
Hyperhomocysteinemia
echinocystic acid
vascular endothelial cells
H&E stain
Biology
03 medical and health sciences
chemistry.chemical_compound
Immunology and Microbiology (miscellaneous)
Adventitia
medicine.artery
Internal medicine
medicine
Vitamin B12
hyperhomocysteinemia
Aorta
General Medicine
Articles
medicine.disease
Endothelial stem cell
030104 developmental biology
Endocrinology
medicine.anatomical_structure
chemistry
Apoptosis
nuclear factor κB
cytochrome P450 1A1
Subjects
Details
- Language :
- English
- ISSN :
- 17921015 and 17920981
- Volume :
- 14
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Experimental and Therapeutic Medicine
- Accession number :
- edsair.doi.dedup.....0af69689e7a285ad711c30b7cf77dac2