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Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue
- Source :
- Molecular Biology Reports. 37:3567-3574
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.
- Subjects :
- Small RNA
Molecular Sequence Data
Sus scrofa
Adipose tissue
Biology
microRNA
Gene expression
RNA Precursors
Genetics
Animals
Cloning, Molecular
Muscle, Skeletal
Molecular Biology
Regulation of gene expression
Genome
Base Sequence
Sequence Analysis, RNA
Myogenesis
cDNA library
Gene Expression Profiling
General Medicine
Molecular biology
Cell biology
MicroRNAs
Adipose Tissue
Gene Expression Regulation
Adipogenesis
Multigene Family
Nucleic Acid Conformation
Subjects
Details
- ISSN :
- 15734978 and 03014851
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Molecular Biology Reports
- Accession number :
- edsair.doi.dedup.....0b0fc63edf9ce173555d0f2ba7b16bd3