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Characterization of a novel microRNA, miR-188, elevated in serum of muscular dystrophy dog model
- Source :
- PLoS ONE, Vol 14, Iss 1, p e0211597 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression at the post-transcriptional level. Several miRNAs are exclusively expressed in skeletal muscle and participate in the regulation of muscle differentiation by interacting with myogenic factors. These miRNAs can be found at high levels in the serum of patients and animal models for Duchenne muscular dystrophy, which is expected to be useful as biomarkers for their clinical conditions. By miRNA microarray analysis, we identified miR-188 as a novel miRNA that is elevated in the serum of the muscular dystrophy dog model, CXMDJ. miR-188 was not muscle-specific miRNA, but its expression was up-regulated in skeletal muscles associated with muscle regeneration induced by cardiotoxin-injection in normal dogs and mice. Manipulation of miR-188 expression using antisense oligo and mimic oligo RNAs alters the mRNA expression of the myogenic regulatory factors, MRF4 and MEF2C. Our results suggest that miR-188 is a new player that participates in the gene regulation process of muscle differentiation and that it may serve as a serum biomarker reflecting skeletal muscle regeneration.
- Subjects :
- 0301 basic medicine
Microarrays
Duchenne muscular dystrophy
Biochemistry
Myoblasts
Mice
0302 clinical medicine
Gene expression
Morphogenesis
Medicine and Health Sciences
Muscular dystrophy
Musculoskeletal System
Cells, Cultured
Mammals
Regulation of gene expression
Multidisciplinary
Muscles
Messenger RNA
Eukaryota
Cell Differentiation
Muscle Differentiation
Cell biology
Nucleic acids
Bioassays and Physiological Analysis
medicine.anatomical_structure
Vertebrates
Medicine
Anatomy
Muscle Regeneration
Research Article
Science
Biology
Research and Analysis Methods
03 medical and health sciences
Dogs
microRNA
Genetics
medicine
Animals
Regeneration
Non-coding RNA
Muscle, Skeletal
Natural antisense transcripts
Organisms
Biology and Life Sciences
Skeletal muscle
Gene Regulation Process
Muscular Dystrophy, Animal
medicine.disease
Gene regulation
Muscular Dystrophy, Duchenne
MicroRNAs
Disease Models, Animal
030104 developmental biology
Skeletal Muscles
Gene Expression Regulation
Amniotes
Myogenic regulatory factors
RNA
Organism Development
Biomarkers
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....0b30a1d6d9b51cf61fa282b7802f6842