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Characterization of the promoter and 5'-UTR intron of oleic acid desaturase (FAD2) gene in Brassica napus
- Source :
- Gene. 545(1)
- Publication Year :
- 2014
-
Abstract
- In the present study, we characterized the transcriptional regulatory region (KF038144) controlling the expression of a constitutive FAD2 in Brassica napus . There are multiple FAD2 gene copies in B. napus genome. The FAD2 gene characterized and analyzed in the study is located on chromosome A5 and was designated as BnFAD 2A5-1. BnFAD 2A5-1 harbors an intron (1192 bp) within its 5′-untranslated region (5′-UTR). This intron demonstrated promoter activity. Deletion analysis of the BnFAD 2A5-1 promoter and intron through the β-glucuronidase (GUS) reporter system revealed that the − 220 to − 1 bp is the minimum promoter region, while − 220 to − 110 bp and + 34 to + 285 bp are two important regions conferring high-levels of transcription. BnFAD 2 transcripts were induced by light, low temperature, and abscisic acid (ABA). These observations demonstrated that not only the promoter but also the intron are involved in controlling the expression of the BnFAD 2A5-1 gene. The intron-mediated regulation is an essential aspect of the gene expression regulation.
- Subjects :
- Fatty Acid Desaturases
Five prime untranslated region
Molecular Sequence Data
Arabidopsis
Gene Dosage
Biology
Genome
chemistry.chemical_compound
Transcription (biology)
Gene Expression Regulation, Plant
Genetics
Promoter Regions, Genetic
Gene
Abscisic acid
Phylogeny
Regulation of gene expression
Base Sequence
Brassica napus
Intron
Temperature
food and beverages
Promoter
General Medicine
Plants, Genetically Modified
Molecular biology
chemistry
5' Untranslated Regions
Abscisic Acid
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 545
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....0b3f0914cce2c084deffc544b9811dc4