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Nucleus pulposus cell senescence is alleviated by resveratrol through regulating the ROS/NF-κB pathway under high-magnitude compression
- Source :
- Bioscience Reports
- Publication Year :
- 2018
- Publisher :
- Portland Press Ltd., 2018.
-
Abstract
- Mechanical overloading is a risk factor of disc degeneration. Studies have demonstrated that resveratrol helps to maintain the disc cell’s healthy biology. The present study aims to investigate whether resveratrol can suppress mechanical overloading-induced nucleus pulposus (NP) cell senescence in vitro and the potential mechanism. The isolated rat NP cells were seeded in the decalcified bone matrix (DBM) and cultured under non-compression (control) and compression (20% deformation, 1.0 Hz, 6 h/day) for 5 days using the mechanically active bioreactor. The resveratrol (30 and 60 μM) was added into the culture medium of the compression group to investigate its protective effects against the NP cell senescence. NP cell senescence was evaluated by cell proliferation, cell cycle, senescence-associated β-galactosidase (SA-β-Gal) activity, telomerase (TE) activity, and gene expression of the senescence markers (p16 and p53). Additionally, the reactive oxygen species (ROS) content and activity of the NF-κB pathway were also analyzed. Compared with the non-compression group, the high-magnitude compression significantly promoted NP cell senescence, increased ROS generation and activity of the NF-κB pathway. However, resveratrol partly attenuated NP cell senescence, decreased ROS generation and activity of the NF-κB pathway in a concentration-dependent manner under mechanical compression. Resveratrol can alleviate mechanical overloading-induced NP cell senescence through regulating the ROS/NF-κB pathway. The present study provides that resveratrol may be a potential drug for retarding mechanical overloading-induced NP cell senescence.
- Subjects :
- Male
Senescence
Nucleus Pulposus
Cell
Biophysics
resveratrol
Resveratrol
Biochemistry
Antioxidants
Rats, Sprague-Dawley
Weight-Bearing
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Molecular Biology
Research Articles
Cells, Cultured
Cellular Senescence
030203 arthritis & rheumatology
chemistry.chemical_classification
Reactive oxygen species
Chemistry
Cell growth
NF-kappa B
NF-κB
Cell Biology
Cell cycle
compression
In vitro
Cell biology
medicine.anatomical_structure
cell senescence
Reactive Oxygen Species
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 15734935 and 01448463
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....0b4077cbfcf41414ca952d58f7a77c46