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Large-volume leukapheresis procedure for peripheral blood progenitor cell collection in children weighing 15 kg or less: Efficacy and safety evaluation

Authors :
François Demeocq
Jean Bernard Palcoux
Jean Louis Stéphan
Jean-Pierre Vannier
Marc G. Berger
Lionel deLumley
Justyna Kanold
Céline Rouzier
Chantal Rapatel
Pascale Halle
Source :
Medical and Pediatric Oncology. 32:7-10
Publication Year :
1999
Publisher :
Wiley, 1999.

Abstract

Background We update our experience on large-volume leukapheresis (LVL) in very small patients with malignancies. LVLs were performed with the aim of reducing the psychological impact of leukaphereses by reducing the number of procedures while collecting large numbers of cells. Procedure Seventeen LVLs were performed using a Cobe Spectra separator in 14 patients weighing ⩽15 kg. A median of 3.8 patient's blood volumes corresponding to 296 mL/kg (range, 202–565) of blood was processed per session of 190 minutes (120–279) duration. A femoral catheter was installed specially for collection for 88% LVL (vs. 35% for standard leukaphereses). A median volume of 16.9 mL/kg was collected with 5.4 × 108 MNC/kg (range, 0.6–16.3) and 8.2 × 106 CD34+ cells/kg (range, 1.3–31.7). Results No signs of complications due to citrate toxicity were encountered. No hypotensive or hypothermic episodes were observed. Platelet counts were significantly diminished after each procedure (median: −59%). When the extracorporal line was not primed with red blood cells (RBC), the difference between pre-LVL and post-LVL hemoglobin levels was significant with a median 32 g/L decrease. Conclusions The LVL approach for peripheral blood progenitor cells (PBPC) collection in very small children may expose them to the risk of anemia and thrombocytopenia and an excess of special central line installation. The application of this technique in these patients should be reserved for special cases when a very large number of cells must be collected and should be performed by an experienced team. Med. Pediatr. Oncol. 32:7–10, 1999. © 1999 Wiley-Liss, Inc.

Details

ISSN :
1096911X and 00981532
Volume :
32
Database :
OpenAIRE
Journal :
Medical and Pediatric Oncology
Accession number :
edsair.doi.dedup.....0b4f063887d150196f08338b1aa1f093
Full Text :
https://doi.org/10.1002/(sici)1096-911x(199901)32:1<7::aid-mpo3>3.0.co;2-z