Back to Search
Start Over
DHA Reduces Oxidative Stress after Perinatal Asphyxia: A Study in Newborn Piglets
- Publication Year :
- 2017
-
Abstract
- Background: Perinatal hypoxic-ischemic brain damage is a major cause of acute mortality and chronic neurological morbidity in infants and children. Oxidative stress due to free radical formation and the initiation of abnormal oxidative reactions appears to play a key role. Docosahexanoic acid (DHA), a main component of brain membrane phospholipids, may act as a neuroprotectant after hypoxia-ischemia by regulating multiple molecular pathways and gene expression. Objectives: The aims of this study were to test the hypothesis that DHA provides significant protection against lipoperoxidation damage in the cerebral cortex and hippocampus in a neonatal piglet model of severe hypoxia-reoxygenation. Methods: Newborn piglets, Noroc (LYLD), were subjected to severe global hypoxia. One group was resuscitated with ambient air (21% group, n = 11) and another also received 5 mg/kg of DHA 4 h after the end of hypoxia (21% DHA group, n = 10). After 9.5 h, tissues from the prefrontal cortex and hippocampus were sampled and the levels of isoprostanes, neuroprostanes, neurofurans, and F2-dihomo-isoprostanes were determined by the liquid chromatography triple quadrupole mass spectrometry technique. Results: Lipid peroxidation biomarkers were significantly lower in both the cortex and hippocampus in the DHA-treated group compared with the untreated group. Conclusions: The present study demonstrates that DHA administration after severe hypoxia in newborn piglets has an antioxidative effect in the brain, suggesting a protective potential of DHA if given after injuries to the brain.
- Subjects :
- 0301 basic medicine
Docosahexaenoic Acids
Neurological morbidity
Sus scrofa
Prefrontal Cortex
Physiology
Oxidative phosphorylation
Brain damage
Isoprostanes
medicine.disease_cause
Hippocampus
Pediatrics
Antioxidants
Docosahexanoic acid
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Neuroprostanes
Brain injury
Lipid metabolism
Newborn infant
Oxidative stress
Pediatrics, Perinatology and Child Health
Developmental Biology
Furans
Asphyxia Neonatorum
business.industry
Perinatology and Child Health
medicine.disease
Infant newborn
Perinatal asphyxia
Disease Models, Animal
Neuroprotective Agents
030104 developmental biology
Animals, Newborn
Anesthesia
Hypoxia-Ischemia, Brain
Lipid Peroxidation
medicine.symptom
business
Biomarkers
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0b9a9fb71edbddcf24737426fdb8dd84