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Quantitative histological assessment of hepatic ischemia-reperfusion injuries following ischemic pre- and post-conditioning in the rat liver
- Source :
- Knudsen, A R, Kannerup, A-S, Grønbæk, H, Hamilton-Dutoit, S J, Nyengaard, J R, Funch-Jensen, P & Mortensen, F V 2013, ' Quantitative histological assessment of hepatic ischemia-reperfusion injuries following ischemic pre-and post-conditioning in the rat liver ', The Journal of surgical research, vol. 180, no. 1, pp. e11-20 . https://doi.org/10.1016/j.jss.2012.03.036, Aarhus University
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Background Ischemic preconditioning (IPC) has been shown to protect the liver against ischemia-reperfusion (I/R) injuries. However, ischemic post-conditioning has received little attention. The aim of the present study was to quantify and compare the hepato-protective properties of IPC and IPO, for the first time, using unbiased design-based stereological methods. Methods We divided 67 rats into four groups: sham, liver ischemia (LI), IPC, and IPO. Rats were subjected to 60 min LI, followed by 4- or 24-h reperfusion. We performed quantification of (NVR) and apoptotic cell profile number. Results We observed no significant differences in NVR between ischemic groups after 4 h. After 24-h reperfusion, NVR had increased to 70% in the LI group, compared with 51% ( P = 0.02) and 49% ( P = 0.01) in the IPC and IPO groups, respectively. After 4-h reperfusion, the apoptotic cell number was significantly higher in all ischemic groups than in the sham group; we detected no difference between ischemic groups. After 24-h reperfusion, we detected a significantly lower number of apoptotic cell profiles in the IPC group than in the LI group ( P = 0.02). The mean number of apoptotic cell profiles decreased insignificantly in the IPO group ( P = 0.06). Liver parameters were at all time comparable between groups. Conclusions After I/R, IPC and IPO reduce the degree of hepatocellular injury. Both methods are equally efficient at preventing hepatocellular necrosis. Furthermore, apoptosis is significantly lower after IPC.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Cell
Urology
Apoptosis
Stereology
medicine
Animals
alpha-Macroglobulins
cardiovascular diseases
Rats, Wistar
Ischemic Postconditioning
Ischemic Preconditioning
Pre and post
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
medicine.disease
Rats
Hepatic ischemia
medicine.anatomical_structure
Liver
Reperfusion Injury
Conditioning
Ischemic preconditioning
Surgery
business
Reperfusion injury
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Journal of Surgical Research
- Accession number :
- edsair.doi.dedup.....0ba8c774548b1ccde04740ee550ffec8