Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia

(A) Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 24 hours. Total cellular DNA were extracted and relative mitochondria/nuclear DNA ratio were measured using Q-PCR. (Data represent Mean {plus minus} S.D of four independent experiments). (B) Kelly cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 6h. ROS levels were measured using a flow cytometry-based MitoTracker Red CM-H2Ros assay. All values are normalized to normoxic shNT control cells (Data represent Mean {plus minus} S.D of three biological repeats, ***p