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Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma
- Source :
- Oncology Reports
- Publication Year :
- 2018
- Publisher :
- D.A. Spandidos, 2018.
-
Abstract
- Nuclear size and shape are important components in the diagnosis of pathological specimens. However, a qualitative evaluation is typically applied rather than a quantitative evaluation technique. In the present study, we sought to evaluate the nuclear morphological characteristics of lung adenocarcinoma using whole-slide imaging (WSI) and computer-assisted image analysis (IA). We evaluated the nuclear characteristics of 106 cases of surgically resected lung adenocarcinoma according to Feulgen staining and immunohistochemistry (IHC) for the inner nuclear membrane protein emerin. According to the Feulgen reaction, although the nuclear area (size) of the carcinoma cells was correlated with the nuclear perimeter (NP) (R=0.8973), the nuclear staining intensity of carcinoma cells was not correlated with the nuclear area. Using emerin IHC, we used IA software that was able to detect both the NP and the emerin-stained nuclear membrane length (ENML) in the nucleus, and found that the more nuclei exhibited a longer ENML relative to the NP, the more nuclear grooves and intranuclear cytoplasmic inclusions were present. In addition, the nuclear area was correlated with the percentage of nuclei that had a longer ENML compared to the NP against the total nuclei (R=0.7759). Furthermore, the emerin low expression group showed an enlarged nuclear area (P=0.0264), elongated NP (P=0.0091), and lower shape factor (P=0.0486) compared with the normal emerin expression group. Our data indicated the usefulness of WSI and IA for pathological specimen analysis. In addition, this study is the first to report that the low expression of emerin in cancer cell results in an oval shape of nuclei and nuclear enlargement in clinical samples.
- Subjects :
- 0301 basic medicine
Male
Cancer Research
Pathology
medicine.medical_specialty
Cytoplasm
Lung Neoplasms
Cytoplasmic inclusion
Emerin
nuclear morphology
Adenocarcinoma of Lung
03 medical and health sciences
0302 clinical medicine
image analysis
medicine
Image Processing, Computer-Assisted
Rosaniline Dyes
Inner membrane
Humans
Feulgen stain
Nuclear membrane
Cell Nucleus
Inclusion Bodies
emerin
Chemistry
Membrane Proteins
Nuclear Proteins
General Medicine
Articles
Middle Aged
medicine.disease
Immunohistochemistry
Single Molecule Imaging
whole slide imaging
Feulgen reaction
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Adenocarcinoma
Female
Nucleus
Subjects
Details
- Language :
- English
- ISSN :
- 17912431 and 1021335X
- Volume :
- 41
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....0bdfdbf84af3f6abbfb4c183bcf8af1c