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Transcriptome analysis reveals that the multiple metabolic pathways were related to gluten polymerization in different quality wheats (Triticum aestivum L.)
- Source :
- Food Science & Nutrition, Food Science & Nutrition, Vol 8, Iss 8, Pp 4573-4583 (2020), Food Science & Nutrition, 8(8):4573-4583
- Publication Year :
- 2019
-
Abstract
- The rapid development of transcriptome sequencing technology has contributed to the discovery of numerous genes in plant; however, the role of gene expression in postharvest wheat remains largely unexplored. In this study, differentially expressed genes (DEGs) were identified by RNA‐seq in different quality wheats. The 102.6 Gb clean reads had been yielded from the nine RNA‐seq libraries. Typically, there were 1791 upregulated and 2,677 downregulated DEGs, respectively, in strong‐gluten wheat compared with weak‐gluten wheat. Specifically, a total of 4,468 DEGs were classified into 286 Gene Ontology (GO) terms and 131 Kyoto Encyclopedia of Genes and Genomes terms (KEGG). Moreover, the storage protein components, starch and sucrose metabolism, and plant hormone signal transduction‐related genes were discovered, which had involved 109 DEGs. The wet gluten proteins content was 35.24% and 17.36%, and the glutenin macropolymer content was 6.38% and 5.01% between the strong‐ and weak‐gluten wheat, respectively. The POD activities of the different quality wheats were 6,571.14, 5,341.24, and 4,851.48 U/g/min, respectively. The significant difference of starch and sucrose metabolism, hormone, POD, and CAT enzyme along with the higher ATPase activity might potentially affect gluten polymerization, which might thereby result in the different qualities of wheats.<br />This work firstly subjected transcriptome to reveal the effect of the different quality wheat, and we found that the formation of different quality wheats may be due to the difference of GMP, storage protein, hormone, protein isomerase, antioxidase activity, and energy metabolism.
- Subjects :
- 0301 basic medicine
lcsh:TX341-641
RNA sequence
Transcriptome
03 medical and health sciences
0404 agricultural biotechnology
Glutenin
wheat
Gene expression
Storage protein
KEGG
Gene
Original Research
chemistry.chemical_classification
biology
transcriptome
gluten polymerization
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
Gluten
030104 developmental biology
chemistry
Biochemistry
biology.protein
Plant hormone
lcsh:Nutrition. Foods and food supply
Food Science
Subjects
Details
- ISSN :
- 20487177
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Food sciencenutrition
- Accession number :
- edsair.doi.dedup.....0bf3e85256ad4f5a99b21263c55a9b31