Back to Search
Start Over
Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504
- Source :
- Molecular Cell. 38:689-699
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Tumor suppressor p53 plays a central role in tumor prevention. p53 protein levels and activity are under a tight and complex regulation in cells to maintain the proper function of p53. MicroRNAs play a key role in the regulation of gene expression. Here we report the regulation of p53 through miR-504. miR-504 acts as a negative regulator of human p53 through its direct binding to two sites in the p53 3' untranslated region. Overexpression of miR-504 decreases p53 protein levels and functions in cells, including p53 transcriptional activity, p53-mediated apoptosis, and cell-cycle arrest in response to stress, and furthermore promotes tumorigenecity of cells in vivo. These results demonstrate the direct negative regulation of p53 by miR-504 as a mechanism for p53 regulation in cells, which highlights the importance of microRNAs in tumorigenesis.
- Subjects :
- Transcription, Genetic
Cell Transplantation
Mice, Nude
Apoptosis
Biology
medicine.disease_cause
Article
law.invention
Mice
03 medical and health sciences
0302 clinical medicine
Genes, Reporter
Stress, Physiological
law
Transcription (biology)
Cell Line, Tumor
microRNA
medicine
Animals
Humans
3' Untranslated Regions
Molecular Biology
030304 developmental biology
Regulation of gene expression
Mice, Inbred BALB C
0303 health sciences
Binding Sites
Three prime untranslated region
Cell Cycle
Cell Biology
Cell cycle
Molecular biology
Cell biology
MicroRNAs
Cell culture
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Suppressor
Female
Tumor Suppressor Protein p53
Carcinogenesis
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....0c20ca752fa3a4721fe20b325e5e025e