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miR-28-5p inhibits cholangiocarcinoma progression and predicts good prognosis of patients
- Source :
- Cell Cycle
- Publication Year :
- 2022
- Publisher :
- Taylor & Francis, 2022.
-
Abstract
- Cholangiocarcinoma (CCA) is one of the most common hepatic and biliary malignancies. The overall five-year survival rate for cholangiocarcinoma is less than 15%. miR-28-5p has been reported to participate the development of various human cancer types. But whether miR-28-5p is associated with the clinical course of CCA patients has not been clarified. Herein, we observed that miR-28-5p was reduced in CCA tissues and predicts the poor prognosis of CCA patients. Treatment with the demethylating agent 5-aza-2’-deoxycytidine (5-AZA) restored miR-28-5p expression in CCA cell lines. Furthermore, up-regulated miR-28-5p inhibited CCA cells growth and metastasis. Mechanistically, miR-28-5p suppressed CCA cells growth and metastasis via directly targeting CD44 molecular. Specific CD44 special siRNA abrogated the discrepancy of the proliferation and metastasis capacity between miR-28-5p-overexpression CCA cells and their control cells, which further confirmed that CD44 was required in miR-28-5p-inhibited CCA cell growth and metastasis.
- Subjects :
- fungi
information science
Cell Biology
Cholangiocarcinoma
Gene Expression Regulation, Neoplastic
MicroRNAs
Bile Ducts, Intrahepatic
Bile Duct Neoplasms
Cell Line, Tumor
parasitic diseases
cardiovascular system
Humans
cardiovascular diseases
Molecular Biology
Cell Proliferation
Research Paper
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Cell Cycle
- Accession number :
- edsair.doi.dedup.....0c2bb2fcfc8dcc7ae1ced4d627ec11bd
- Full Text :
- https://doi.org/10.6084/m9.figshare.20015193.v1