Back to Search
Start Over
The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2010
- Publisher :
- Dove Medical Press, 2010.
-
Abstract
- Bao-An Chen1, Nan Jin1, Jun Wang1, Jiahua Ding1, Chong Gao1, Jian Cheng1, Guohua Xia1, Feng Gao1, Yin Zhou1, Yue Chen1, Guina Zhou1, Xiaomao Li2, Yu Zhang3, Men Tang3, Xuemei Wang31Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People’s Republic of China; 2Department of Physics, University of Saarland, D-266041 Saarbruechen, Germany; 3National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, People’s Republic of ChinaAbstract: We investigated the effect of magnetic nanoparticles of Fe3O4 (Fe3O4-MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe3O4-MNPs, respectively. After intravenous administration of Fe3O4-MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P > 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P < 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P < 0.05). In peripheral blood, both the proportions of subset CD4+ and CD8+ T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4+ T cells between the medium- and low-dose groups. Interestingly, the Fe3O4-MNPs enhanced the production of interleukin-2 (IL-2), interferon-γ, and IL-10 but did not affect the production of IL-4 in peripheral blood. It is concluded that Fe3O4-MNPs could influence immune functions of normal ICR mice in a dose-dependent manner.Keywords: magnetic nanoparticle of Fe3O4, immune function, splenocyte proliferation, cytokine
- Subjects :
- CD4-Positive T-Lymphocytes
Male
medicine.medical_specialty
medicine.medical_treatment
Biophysics
Pharmaceutical Science
Bioengineering
Spleen
magnetic nanoparticle of Fe3O4
Biology
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Flow cytometry
Biomaterials
Mice
Immune system
Microscopy, Electron, Transmission
International Journal of Nanomedicine
Immunity
Internal medicine
Drug Discovery
medicine
Splenocyte
cytokine
Animals
Particle Size
Magnetite Nanoparticles
Saline
Original Research
immune function
Mice, Inbred ICR
medicine.diagnostic_test
Dose-Response Relationship, Drug
splenocyte proliferation
Organic Chemistry
General Medicine
Endocrinology
medicine.anatomical_structure
Cytokine
Nanomedicine
Immunology
Cytokines
Female
CD8
Subjects
Details
- Language :
- English
- ISSN :
- 11782013 and 11769114
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....0c61e97bd08e328ae15ad8d9a8b79fb9