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Field evaluation of two commercial RT-rtPCR assays for porcine reproductive and respiratory syndrome virus detection using sera from ill and healthy pigs, China
- Source :
- Journal of Veterinary Diagnostic Investigation. 30:848-854
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious respiratory virus causing severe morbidity in pigs worldwide. Control strategies for PRRSV often rely on detecting PRRSV, culling or isolating sick pigs, disinfecting pig barns, vaccination, and monitoring for virus spread. Given the high economic impact of PRRSV on pig farms, there is a great need for rapid and reliable PRRSV detection assays. We compared the performance of 2 commercial reverse-transcription real-time PCR (RT-rtPCR) assays, the VetMAX PRRSV NA and EU reagents (ABI assay) and the PRRSV general RT-rtPCR kit (Anheal assay), for the molecular detection of PRRSV in sera collected from pigs in China. Between June and September 2015, sera were collected from 219 healthy and 104 suspected PRRSV-infected pigs on 4 farms in China. Employing blinding, the 2 assays were run by 2 laboratories (Guangzhou Animal Health Inspection Institute [GAHII] and Sun Yat-sen University [SYSU] laboratories) and compared. Although both assays detected PRRSV with 100% specificity at both laboratories, the sensitivity (95% vs. 78% at GAHII; 94% vs. 72% at SYSU Laboratory) and the reproducibility (kappa value 0.933 vs. 0.931) were slightly better for the ABI assay compared to the Anheal assay.
- Subjects :
- Male
0301 basic medicine
China
Swine
viruses
animal diseases
Porcine Reproductive and Respiratory Syndrome
Culling
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Virus
Serology
03 medical and health sciences
Blood serum
Animals
Pig farming
Medicine
Porcine respiratory and reproductive syndrome virus
Full Scientific Reports
General Veterinary
biology
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Reproducibility of Results
virus diseases
respiratory system
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
Virology
Vaccination
030104 developmental biology
Respiratory virus
Female
business
Subjects
Details
- ISSN :
- 19434936 and 10406387
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Diagnostic Investigation
- Accession number :
- edsair.doi.dedup.....0c62f7f5db4410af9c84f7a5b9730568