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Correction to: MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1
- Source :
- Protein & Cell
- Publication Year :
- 2021
- Publisher :
- Higher Education Press, 2021.
-
Abstract
- MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role of non-protein-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer (CRC) and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phenocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potential tumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.
- Subjects :
- Male
Colorectal cancer
Down-Regulation
Mice, Nude
Biology
Biochemistry
Mir 139 5p
Text mining
Cell Movement
Cell Line, Tumor
Sequence Homology, Nucleic Acid
Drug Discovery
medicine
Animals
Humans
Neoplasm Invasiveness
Receptor, Notch1
Mice, Inbred BALB C
Base Sequence
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Correction
Cell Biology
Middle Aged
medicine.disease
HCT116 Cells
Survival Analysis
Xenograft Model Antitumor Assays
Human genetics
Gene Expression Regulation, Neoplastic
Receptors, Autocrine Motility Factor
MicroRNAs
HEK293 Cells
Cancer research
Female
RNA Interference
Stem cell
business
Colorectal Neoplasms
Developmental biology
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 16748018 and 1674800X
- Volume :
- 12
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Protein & Cell
- Accession number :
- edsair.doi.dedup.....0c6ce114c54e3e97ce8131cccf6c72dc