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Single-cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics
- Source :
- Cell Proliferation
- Publication Year :
- 2020
-
Abstract
- Objectives Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single‐cell RNA sequencing to thoroughly characterize mouse bladder urothelium. Materials and methods A total of 18,917 single cells from mouse bladder urothelium were analysed by unbiased single‐cell RNA sequencing. The expression of the novel cell marker was confirmed by immunofluorescence using urinary tract infection models. Results Unsupervised clustering analysis identified 8 transcriptionally distinct cell subpopulations from mouse bladder urothelial cells. We discovered a novel type of bladder urothelial cells marked by Plxna4 that may be involved with host response and wound healing. We also found a group of basal‐like cells labelled by ASPM that could be the progenitor cells of adult bladder urothelium. ASPM+ urothelial cells are significantly increased after injury by UPEC. In addition, specific transcription factors were found to be associated with urothelial cell differentiation. At the last, a number of interstitial cystitis/bladder pain syndrome–regulating genes were found differentially expressed among different urothelial cell subpopulations. Conclusions Our study provides a comprehensive characterization of bladder urothelial cells, which is fundamental to understanding the biology of bladder urothelium and associated bladder disease.<br />Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single‐cell RNA sequencing to thoroughly characterize mouse bladder urothelium. A total of 18 917 single cells from mouse bladder urothelium were analysed by unbiased single‐cell RNA sequencing. The expression of the novel cell marker was confirmed by immunofluorescence using urinary tract infection models. Unsupervised clustering analysis identified 8 transcriptionally distinct cell subpopulations from mouse bladder urothelial cells. We discovered a novel type of bladder urothelial cells marked by Plxna4 that may be involved with host response and wound healing. We also found a group of basal‐like cells labelled by ASPM that could be the progenitor cells of adult bladder urothelium. ASPM+ urothelial cells are significantly increased after injury by UPEC. In addition, specific transcription factors were found to be associated with urothelial cell differentiation. At the last, a number of interstitial cystitis/bladder pain syndrome–regulating genes were found differentially expressed among different urothelial cell subpopulations. Our study provides a comprehensive characterization of bladder urothelial cells, which is fundamental to understanding the biology of bladder urothelium and associated bladder disease.
- Subjects :
- 0301 basic medicine
Cell type
Urothelial Cell
Urinary Bladder
Nerve Tissue Proteins
Receptors, Cell Surface
Biology
urologic and male genital diseases
bladder urothelium
Bladder Urothelium
Transcriptome
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
Cell Lineage
Urothelium
Progenitor cell
Bladder Pain
Sequence Analysis, RNA
Stem Cells
Interstitial cystitis
Cell Differentiation
Cell Biology
General Medicine
Original Articles
cell subtype
medicine.disease
female genital diseases and pregnancy complications
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
030220 oncology & carcinogenesis
Urinary Tract Infections
Cancer research
Calmodulin-Binding Proteins
Female
Original Article
Single-Cell Analysis
single‐cell transcriptome
Biomarkers
Transcription Factors
Subjects
Details
- ISSN :
- 13652184
- Volume :
- 54
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cell proliferation
- Accession number :
- edsair.doi.dedup.....0c7be0042721e159ec6fdcd71b97f1f8