Back to Search
Start Over
Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle
- Source :
- Veterinary Research, Veterinary Research, BioMed Central, 2019, 50 (1), pp.99. ⟨10.1186/s13567-019-0720-5⟩, Veterinary Research, Vol 50, Iss 1, Pp 1-11 (2019), Vohra, P, Vrettou, C, Hope, J, Hopkins, J & Stevens, M 2019, ' Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle ', Veterinary Research, pp. 99 . https://doi.org/10.1186/s13567-019-0720-5
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Salmonella entericais a veterinary and zoonotic pathogen of global importance. While murine and cell-based models of infection have provided considerable knowledge about the molecular basis of virulence ofSalmonella, relatively little is known about salmonellosis in naturally-affected large animal hosts such as cattle, which are a reservoir of human salmonellosis. As in humans,Salmonellacauses bovine disease ranging from self-limiting enteritis to systemic typhoid-like disease and exerts significant economic and welfare costs. Understanding the nature and consequences ofSalmonellainteractions with bovine cells will inform the design of effective vaccines and interventions to control animal and zoonotic infections. In calves challenged orally withS. Dublin expressing green fluorescent protein (GFP) we observed that the bacteria were predominantly extracellular in the distal ileal mucosa and within gut-associated lymph nodes 48 h post-infection. Intracellular bacteria, identified by flow cytometry using the GFP signal, were predominantly within MHCII+macrophage-like cells. In contrast to observations from murine models, theseS.Dublin-infected cells had elevated levels of MHCII and CD40 compared to both uninfected cells from the same tissue and cells from the cognate tissue of uninfected animals. Moreover, no gross changes of the architecture of infected lymph nodes were observed as was described previously in a mouse model. In order to further investigateSalmonella-macrophage interactions, net replication ofS. entericaserovars that differ in virulence in cattle was measured in bovine blood-derived macrophages by enumeration of gentamicin-protected bacteria and fluorescence dilution, but did not correlate with host-specificity.
- Subjects :
- 0301 basic medicine
Serotype
Salmonella
[SDV]Life Sciences [q-bio]
030106 microbiology
Cattle Diseases
Virulence
Serogroup
medicine.disease_cause
Major histocompatibility complex
Microbiology
03 medical and health sciences
medicine
Animals
2. Zero hunger
Salmonella Infections, Animal
response
lcsh:Veterinary medicine
CD40
General Veterinary
biology
Zoonotic Infection
tropism
Intracellular parasite
Histocompatibility Antigens Class II
Salmonella enterica
biology.organism_classification
3. Good health
030104 developmental biology
cattle
biology.protein
cells
lcsh:SF600-1100
Cattle
host-specificity
MHC
Research Article
Subjects
Details
- ISSN :
- 12979716 and 09284249
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Veterinary Research
- Accession number :
- edsair.doi.dedup.....0c8809ff00c03681494a843bf417907e
- Full Text :
- https://doi.org/10.1186/s13567-019-0720-5