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Bovine Serum Albumin Protein-Based Liquid Crystal Biosensors for Optical Detection of Toxic Heavy Metals in Water
- Source :
- Sensors (Basel, Switzerland), Sensors, Volume 20, Issue 1, Sensors, Vol 20, Iss 1, p 298 (2020)
- Publication Year :
- 2019
-
Abstract
- A new methodology involving the use of Bovine Serum Albumin (BSA) as a probe and liquid crystal (LC) as a signal reporter for the detection of heavy metal ions in water at neutral pH was developed. BSA acted as a multi-dentate ligand for the detection of multiple metal ions. The LC sensor was fabricated by immobilizing 3 &micro<br />g mL&minus<br />1 BSA solution on dimethyloctadecyl-[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP)-coated glass slides. In the absence of heavy metal ions, a dark optical image was observed, while in their presence, a dark optical image turned to bright. The optical response was characterized by using a polarized optical microscope (POM). The BSA based LC sensor selectively detected toxic metal ions as compared to s block metal ions and ammonium ions in water. Moreover, the limit of detection was found to be very low (i.e., 1 nM) for the developed new biosensor in comparison to reported biosensors.
- Subjects :
- genetic structures
Metal ions in aqueous solution
heavy metal ions
02 engineering and technology
Biosensing Techniques
lcsh:Chemical technology
010402 general chemistry
polarized optical microscope
01 natural sciences
Biochemistry
Article
Analytical Chemistry
Ion
chemistry.chemical_compound
liquid crystals
Liquid crystal
Metals, Heavy
bovine serum albumin
Animals
Humans
lcsh:TP1-1185
Electrical and Electronic Engineering
Bovine serum albumin
Instrumentation
Detection limit
biology
Ligand
Water
Serum Albumin, Bovine
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
0104 chemical sciences
chemistry
biology.protein
Ammonium chloride
Cattle
sense organs
0210 nano-technology
Biosensor
Nuclear chemistry
Subjects
Details
- ISSN :
- 14248220
- Volume :
- 20
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Sensors (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....0c9786c2b90257049bab14409f20fb39