Back to Search
Start Over
Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 18, Iss 10, p 2024 (2017), International Journal of Molecular Sciences; Volume 18; Issue 10; Pages: 2024
- Publication Year :
- 2017
- Publisher :
- MDPI, 2017.
-
Abstract
- The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type (RS-2) and 25 T6SS mutants, which were constructed using homologous recombination methods. The mutation of 15 T6SS genes significantly reduced bacterial virulence and the secretion of Hcp protein. Additionally, the complemented 7 mutations ΔpppA, ΔclpB, Δhcp, ΔdotU, ΔicmF, ΔimpJ, and ΔimpM caused similar virulence characteristics as RS-2. Moreover, the mutant ΔpppA, ΔclpB, ΔicmF, ΔimpJ and ΔimpM genes caused by a 38.3~56.4% reduction in biofilm formation while the mutants ΔpppA, ΔclpB, ΔicmF and Δhcp resulted in a 37.5~44.6% reduction in motility. All together, these results demonstrate that T6SS play vital roles in the virulence of strain RS-2, which may be partially attributed to the reductions in Hcp secretion, biofilm formation and motility. However, differences in virulence between strain RS-1 and RS-2 suggest that other factors may also be involved in the virulence of Aaa.
- Subjects :
- 0301 basic medicine
Iron-Sulfur Proteins
gene knock-out
Mutant
Acidovorax avenae subsp. Avenae
biofilm
lcsh:Chemistry
Gene cluster
pathogenicity
Pathogen
lcsh:QH301-705.5
Spectroscopy
Virulence
Acidovorax
Hcp
General Medicine
Type VI Secretion Systems
Computer Science Applications
Anti-Bacterial Agents
Complementation
T6SS
motility
Multigene Family
growth
030106 microbiology
Biology
Catalysis
Article
Microbiology
Inorganic Chemistry
Comamonadaceae
03 medical and health sciences
Bacterial Proteins
Secretion
Physical and Theoretical Chemistry
Molecular Biology
Type VI secretion system
Plant Diseases
Organic Chemistry
Genetic Complementation Test
Oryza
Gene Expression Regulation, Bacterial
biology.organism_classification
Mutagenesis, Insertional
lcsh:Biology (General)
lcsh:QD1-999
Biofilms
Genome, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....0ca54e233f16b5a07290ada09480e11f