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Multiple locus variable number tandem repeat analysis for the characterization of wild feline Bartonella species and subspecies
- Source :
- Veterinary Microbiology, Veterinary Microbiology, Elsevier, 2020, 247, ⟨10.1016/j.vetmic.2020.108788⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Bartonella genus includes an increasing number of species and subspecies, especially among wild felids, the positioning of which, with regards to the zoonotic species Bartonella henselae, is important to determine.The aim of this study was to test the ability of a molecular typing technique to distinguish between various Bartonella isolates obtained from four different species of free-ranging and captive wild felids and to identify key profiles or markers allowing differentiating them from each other and/or from B. henselae or B. koehlerae.A molecular typing technique for B. henselae based on the polymorphism of variable number tandem repeat units (VNTR) called MLVA (Multiple Locus VNTR Analysis) was applied to 24 Bartonella isolates from free-ranging or captive wild felids, 19 of which were obtained from California and five from three countries in Southern Africa, and compared with 49 B. henselae isolates from cats, dog or humans from the United States including the human ATCC (American Type Culture Collection) reference strain, B. henselae Houston 1.MLVA allowed distinguishing Bartonella isolates from wild felids from either B. henselae or B. koehlerae. We confirmed infection of semi-captive cheetahs with an isolate similar to a Californian bobcat isolate. MLVA also confirmed the unique profile of a free-ranging cheetah isolate from Namibia.Specific profiles were observed making MVLA a useful identification/classification tool of these wild felid isolates and suggesting that they are highly adapted to a specific feline reservoir. Finally, circulation of B. henselae isolates between domestic cats, wild felids and humans is likely occurring, based on the close allelic profiles of some isolates.
- Subjects :
- Bartonella
Animals, Wild
Minisatellite Repeats
Multiple Loci VNTR Analysis
Subspecies
Cat Diseases
Microbiology
California
South Africa
03 medical and health sciences
Dogs
Genus
Bartonella Infections
Animals
Humans
MESH: Animals, Wild
Dog Diseases
Allele
Phylogeny
Disease Reservoirs
030304 developmental biology
Genetics
0303 health sciences
MESH: Disease Reservoirs
Bartonella henselae
Wild felids
General Veterinary
biology
MESH: Bartonella Infections
030306 microbiology
Strain (biology)
MLVA
General Medicine
biology.organism_classification
bacterial infections and mycoses
Namibia
Bacterial Typing Techniques
Variable number tandem repeat
MESH: Bacterial Typing Techniques / methods
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Minisatellite Repeats
Cats
Subjects
Details
- Language :
- English
- ISSN :
- 03781135
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology, Veterinary Microbiology, Elsevier, 2020, 247, ⟨10.1016/j.vetmic.2020.108788⟩
- Accession number :
- edsair.doi.dedup.....0cb91456a39b7635e22e8294b6ef8990