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Low-cost and highly efficient DNA biosensor for heavy metal ion using specific DNAzyme-modified microplate and portable glucometer-based detection mode
- Source :
- Biosensors and Bioelectronics. 68:232-238
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- A simple and low-cost DNA sensing platform based on Pb 2+ -specific DNAzyme-modified microplate was successfully developed for highly sensitive monitoring of lead ion (Pb 2+ , one kind of toxic heavy metal ion) in the environmental samples coupling with a portable personal glucometer (PGM)-based detection mode. The detection cell was first prepared simply by means of immobilizing the DNAzyme on the streptavidin-modified microplate. Gold nanoparticle labeled with single-stranded DNA and invertase (Enz-AuNP-DNA) was utilized as the signal-transduction tag to produce PGM substrate (glucose). Upon addition of lead ion into the microplate, the substrate strand of the immobilized DNAzyme was catalytically cleaved by target Pb 2+ , and the newly generated single-strand DNA in the microplate could hybridize again with the single-stranded DNA on the Enz-AuNP-DNA. Accompanying with the Enz-AuNP-DNA, the carried invertase could convert sucrose into glucose. The as-produced glucose could be monitored by using a widely accessible PGM for in situ amplified digital readout. Based on Enz-AuNP-DNA amplification strategy, as low as 1.0 pM Pb 2+ could be detected under the optimal conditions. Moreover, the methodology also showed good reproducibility and high selectivity toward target Pb 2+ against other metal ions because of highly specific Pb 2+ -dependent DNAzyme, and was applicable for monitoring Pb 2+ in the naturally contaminated sewage and spiked drinking water samples.
- Subjects :
- Metal ions in aqueous solution
Biomedical Engineering
Biophysics
Deoxyribozyme
Analytical chemistry
DNA, Single-Stranded
Metal Nanoparticles
Nanoparticle
Biosensing Techniques
Metal
chemistry.chemical_compound
Electrochemistry
Ions
Chromatography
Chemistry
Blood Glucose Self-Monitoring
Nucleic Acid Hybridization
Substrate (chemistry)
DNA, Catalytic
General Medicine
Invertase
Lead
visual_art
visual_art.visual_art_medium
Gold
Biosensor
DNA
Environmental Monitoring
Biotechnology
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....0ce51b7a7b75a382e73a7108ee3f277c