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First genetic characterization of Toxoplasma gondii infection in common quails (Coturnix coturnix) intended for human consumption in China
- Source :
- Infection, Genetics and Evolution. 49:14-16
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Toxoplasma gondii is widely distributed in humans and other animals including birds throughout the world. However, little is known of the molecular epidemiology and genotypes of T. gondii infecting quails in China. Therefore, the present study was conducted to characterize T. gondii genotypes in common quails in China. During December 2014 to October 2015, a total of 390 muscle tissue samples of common quails were collected and the T. gondii B1 gene was amplified using a nested PCR, and the positive samples were genotyped at 11 genetic markers (SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Twenty-five of the 390 common quails (6.41%) were tested positive by nested PCR. Three DNA samples from the 25 positive samples were typed completely and were identified as ToxoDB Genotype #9 ( http://toxodb.org/toxo/ ). The results of the present study indicated that T. gondii infection in common quails was common in China, which provided the information of T. gondii genetic diversity in this host species. This is the first genetic characterization of T. gondii isolates from quails in China, which is useful for monitoring and controlling the T. gondii infection in quails, other animals and humans.
- Subjects :
- Genetic Markers
0301 basic medicine
Microbiology (medical)
China
Meat
Genotype
Protozoan Proteins
Coturnix
Polymerase Chain Reaction
Microbiology
03 medical and health sciences
Polymorphism (computer science)
parasitic diseases
Genetics
Animals
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Molecular Epidemiology
Genetic diversity
biology
Molecular epidemiology
Bird Diseases
Genetic Variation
Toxoplasma gondii
DNA, Protozoan
biology.organism_classification
Virology
Toxoplasmosis, Animal
030104 developmental biology
Infectious Diseases
Genetic marker
Epidemiological Monitoring
Coturnix coturnix
Toxoplasma
Nested polymerase chain reaction
Polymorphism, Restriction Fragment Length
Subjects
Details
- ISSN :
- 15671348
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Infection, Genetics and Evolution
- Accession number :
- edsair.doi.dedup.....0d01db96ab681f86ac8bb9e82d7dcc66