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Wen-pi-tang-Hab-Wu-ling-san reduces ureteral obstructive renal fibrosis by the reduction of oxidative stress, inflammation, and TGF-beta/Smad2/3 signaling
- Source :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 48(2)
- Publication Year :
- 2009
-
Abstract
- Kidney fibrosis results in chronic renal disease. The current treatment of chronic renal diseases is limited to angiotensin converting enzyme inhibitors and angiotensin receptor blockers. Recently, we found that Wen-pi-tang-Hab-Wu-ling-san (WHW) extract, which has been used to treat renal diseases in herbal medicine for a long time, plays anti-fibrogenic. Here, we investigated the role of WHW in the kidney fibrosis induced by unilateral ureteral obstruction (UUO) in mice. C57BL/6 male mice were subjected to UUO on day 0 and then administered with either WHW (2, 10, or 50 mg/kg of body weight) or vehicle orally from 1 day after UUO to finish the experiment. WHW-administration significantly mitigated the UUO-induced kidney fibrotic changes including tubular atrophy and dilatation, collagen accumulation, expansion of interstitial space and leukocyte infiltration. WHW prevented the increases of oxidative stress by the prevention of UUO-induced decreases of catalase, copper–zinc superoxide dismutase (CuZnSOD) and manganese superoxide dismutase (MnSOD), resulting in reduced production of oxidative stress. Furthermore, WHW reduced transforming growth factor-β (TGF-β) expression and phosphorylation of Smad2/3 stimulated by UUO. In conclusion, WHW prevented kidney fibrosis following UUO by the inhibition of inflammation, oxidative stress and TGF-β/Smad2/3 signaling pathway.
- Subjects :
- Male
medicine.medical_specialty
Inflammation
Smad2 Protein
urologic and male genital diseases
Toxicology
medicine.disease_cause
Kidney
Thiobarbituric Acid Reactive Substances
Antioxidants
Superoxide dismutase
Mice
Fibrosis
Transforming Growth Factor beta
Internal medicine
medicine
Renal fibrosis
Animals
Smad3 Protein
biology
urogenital system
business.industry
Angiotensin-converting enzyme
General Medicine
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
Oxidative Stress
Endocrinology
medicine.anatomical_structure
biology.protein
Lipid Peroxidation
medicine.symptom
Ureter
business
Oxidoreductases
Oxidative stress
Food Science
Kidney disease
Drugs, Chinese Herbal
Signal Transduction
Ureteral Obstruction
Subjects
Details
- ISSN :
- 18736351
- Volume :
- 48
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Accession number :
- edsair.doi.dedup.....0d0b630d04cbb09a0381639bc8cc0be2