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Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019), Scientific Reports, Scientific reports, vol 9, iss 1
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Animal studies have demonstrated beneficial effects of therapeutic hypothermia on myocardial function, yet exact mechanisms remain unclear. Impaired autophagy leads to heart failure and mitophagy is important for mitigating ischemia/reperfusion injury. This study aims to investigate whether the beneficial effects of therapeutic hypothermia are due to preserved autophagy and mitophagy. Under general anesthesia, the left anterior descending coronary artery of 19 female farm pigs was occluded for 90 minutes with consecutive reperfusion. 30 minutes after reperfusion, we performed pericardial irrigation with warm or cold saline for 60 minutes. Myocardial tissue analysis was performed one and four weeks after infarction. Therapeutic hypothermia induced a significant increase in autophagic flux, mitophagy, mitochondrial mass and function in the myocardium after infarction. Cell stress, apoptosis, inflammation as well as fibrosis were reduced, with significant preservation of systolic and diastolic function four weeks post infarction. We found similar biochemical changes in human samples undergoing open chest surgery under hypothermic conditions when compared to the warm. These results suggest that autophagic flux and mitophagy are important mechanisms implicated in cardiomyocyte recovery after myocardial infarction under hypothermic conditions. New therapeutic strategies targeting these pathways directly could lead to improvements in prevention of heart failure.
- Subjects :
- 0301 basic medicine
Aging
Swine
Infarction
lcsh:Medicine
Apoptosis
Hypothermia
Cardiovascular
0302 clinical medicine
Fibrosis
Hypothermia, Induced
Mitophagy
2.1 Biological and endogenous factors
Myocardial infarction
Aetiology
lcsh:Science
2. Zero hunger
Multidisciplinary
3. Good health
Mitochondria
Heart Disease
Treatment Outcome
Echocardiography
Cardiology
Female
Cardiac regeneration
medicine.symptom
medicine.medical_specialty
Ischemia
Myocardial Reperfusion Injury
Heart failure
Article
03 medical and health sciences
Internal medicine
medicine
Autophagy
Animals
Humans
Heart Disease - Coronary Heart Disease
Animal
business.industry
Induced
lcsh:R
medicine.disease
Disease Models, Animal
030104 developmental biology
Disease Models
lcsh:Q
business
Reperfusion injury
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....0d14f14abe1be55c52246a95fca7f41d
- Full Text :
- https://doi.org/10.1038/s41598-019-46452-w