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Correction to: PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling
- Source :
- Genome Medicine, Genome Medicine, Vol 13, Iss 1, Pp 1-2 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Aberrant changes in epigenetic mechanisms such as histone modifications play an important role in cancer progression. PRMT1 which triggers asymmetric dimethylation of histone H4 on arginine 3 (H4R3me2a) is upregulated in human colorectal cancer (CRC) and is essential for cell proliferation. However, how this dysregulated modification might contribute to malignant transitions of CRC remains poorly understood.In this study, we integrated biochemical assays including protein interaction studies and chromatin immunoprecipitation (ChIP), cellular analysis including cell viability, proliferation, colony formation, and migration assays, clinical sample analysis, microarray experiments, and ChIP-Seq data to investigate the potential genomic recognition pattern of H4R3me2s in CRC cells and its effect on CRC progression.We show that PRMT1 and SMARCA4, an ATPase subunit of the SWI/SNF chromatin remodeling complex, act cooperatively to promote colorectal cancer (CRC) progression. We find that SMARCA4 is a novel effector molecule of PRMT1-mediated H4R3me2a. Mechanistically, we show that H4R3me2a directly recruited SMARCA4 to promote the proliferative, colony-formative, and migratory abilities of CRC cells by enhancing EGFR signaling. We found that EGFR and TNS4 were major direct downstream transcriptional targets of PRMT1 and SMARCA4 in colon cells, and acted in a PRMT1 methyltransferase activity-dependent manner to promote CRC cell proliferation. In vivo, knockdown or inhibition of PRMT1 profoundly attenuated the growth of CRC cells in the C57BL/6 J-ApcPRMT1-mediated H4R3me2a recruits SMARCA4, which promotes colorectal cancer progression by enhancing EGFR signaling.
- Subjects :
- Protein-Arginine N-Methyltransferases
Transcription, Genetic
Colorectal cancer
Systems biology
MEDLINE
QH426-470
Arginine
Methylation
Models, Biological
Histones
Mice
Cell Line, Tumor
Tensins
Genetics
Medicine
Animals
Humans
Egfr signaling
Author Correction
Molecular Biology
Genetics (clinical)
Cell Proliferation
business.industry
Dextran Sulfate
DNA Helicases
Nuclear Proteins
medicine.disease
Prognosis
Human genetics
Up-Regulation
ErbB Receptors
Mice, Inbred C57BL
Repressor Proteins
Cancer research
SMARCA4
Disease Progression
Molecular Medicine
business
Colorectal Neoplasms
Signal Transduction
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 1756994X
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Genome Medicine
- Accession number :
- edsair.doi.dedup.....0d1e498e5847ce76fa8132b9301d5acc