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Blocking Notch1 signaling by RNA interference can induce growth inhibition in HeLa cells

Authors :
Shengfang Ge
Guanxiang Qian
Shenglin Huang
Hai Yu
L. Jian
Xiaoping Zhao
Source :
International Journal of Gynecologic Cancer. 17:511-516
Publication Year :
2007
Publisher :
BMJ, 2007.

Abstract

The Notch proteins constitute a family of transmembrane receptors that play a pivotal role in cellular differentiation, proliferation, and apoptosis. RNA interference of Presenilin1 (PS1) and Notch1 was carried out in this research to determine whether it could block Notch signaling and induce growth inhibition in HeLa cells. We transfected synthesized target small interfering RNA (siRNA) into HeLa cells, and blocking of Notch signaling was detected by C-promoter binding factor-1 (CBF1) reporter. We then conducted cell proliferation assay. Cells transfected with PS1 and Notch1 siRNA showed great inhibition in proliferation compared to the controlsin vitroandin vivo. We conclude that RNA interference of PS1 or Notch1 can block Notch signaling and consequently induce growth inhibition of HeLa cells.

Details

ISSN :
15251438 and 1048891X
Volume :
17
Database :
OpenAIRE
Journal :
International Journal of Gynecologic Cancer
Accession number :
edsair.doi.dedup.....0d2a5a290330e8427e2a0f7725998656
Full Text :
https://doi.org/10.1111/j.1525-1438.2007.00813.x