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Blocking Notch1 signaling by RNA interference can induce growth inhibition in HeLa cells
- Source :
- International Journal of Gynecologic Cancer. 17:511-516
- Publication Year :
- 2007
- Publisher :
- BMJ, 2007.
-
Abstract
- The Notch proteins constitute a family of transmembrane receptors that play a pivotal role in cellular differentiation, proliferation, and apoptosis. RNA interference of Presenilin1 (PS1) and Notch1 was carried out in this research to determine whether it could block Notch signaling and induce growth inhibition in HeLa cells. We transfected synthesized target small interfering RNA (siRNA) into HeLa cells, and blocking of Notch signaling was detected by C-promoter binding factor-1 (CBF1) reporter. We then conducted cell proliferation assay. Cells transfected with PS1 and Notch1 siRNA showed great inhibition in proliferation compared to the controlsin vitroandin vivo. We conclude that RNA interference of PS1 or Notch1 can block Notch signaling and consequently induce growth inhibition of HeLa cells.
- Subjects :
- Male
Small interfering RNA
Cellular differentiation
Notch signaling pathway
Mice, Nude
Injections, Intralesional
Biology
Transfection
HeLa
Mice
chemistry.chemical_compound
Genes, Reporter
RNA interference
Presenilin-1
Animals
Humans
RNA, Small Interfering
Receptor, Notch1
Cell Proliferation
Obstetrics and Gynecology
biology.organism_classification
Xenograft Model Antitumor Assays
Molecular biology
Cell biology
Oncology
chemistry
Notch proteins
RNA Interference
Growth inhibition
HeLa Cells
Subjects
Details
- ISSN :
- 15251438 and 1048891X
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Journal of Gynecologic Cancer
- Accession number :
- edsair.doi.dedup.....0d2a5a290330e8427e2a0f7725998656
- Full Text :
- https://doi.org/10.1111/j.1525-1438.2007.00813.x