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A surface-layer protein from Lactobacillus acidophilus NCFM induces autophagic death in HCT116 cells requiring ROS-mediated modulation of mTOR and JNK signaling pathways
- Source :
- Foodfunction. 10(7)
- Publication Year :
- 2019
-
Abstract
- A surface-layer protein (Slp) derived from Lactobacillus acidophilus NCFM has been reported to possess multiple biological properties, including anti-inflammatory, inhibition of apoptosis in pathogen-invaded HT-29 cells and oxidative stress relief. However, its anti-tumor ability and underlying molecular mechanism are unknown. Here, we report that Slp suppresses cell proliferation and induces autophagic cell death in HCT116 cells. Accumulation of Beclin-1 and microtubule-associated protein 1 light chain 3 from II (LC3-II), and the degradation of p62 were observed when cells were treated with various concentrations of Slp (25, 50, 100 μg mL-1) for 24 h. We also found that the mammalian targets of rapamycin (mTOR) and c-Jun N-terminal kinase (JNK) signaling pathways were crucial mediators regulating Slp-induced autophagic cell death. Additionally, treatment with Slp resulted in the obvious formation of reactive oxygen species (ROS). SP600125, a JNK inhibitor, and N-acetylcysteine (NAC), a ROS inhibitor, attenuated Slp-induced autophagic cell death in HCT116 cells. Furthermore, NAC was found to prevent Slp-induced p70 and JNK phosphorylation. Taken together, our results suggest a novel mechanism of action of Slp induced autophagy, acting simultaneously through the ROS-mediated mTOR and JNK signaling pathways in HCT116 colon cancer cells.
- Subjects :
- 0301 basic medicine
Programmed cell death
Cell Survival
MAP Kinase Signaling System
Autophagic Cell Death
P70-S6 Kinase 1
Apoptosis
03 medical and health sciences
Cell Line, Tumor
Autophagy
Humans
PI3K/AKT/mTOR pathway
Cell Proliferation
Anthracenes
030109 nutrition & dietetics
Cell growth
Chemistry
Kinase
TOR Serine-Threonine Kinases
JNK Mitogen-Activated Protein Kinases
Membrane Proteins
General Medicine
HCT116 Cells
Cell biology
Lactobacillus acidophilus
030104 developmental biology
Beclin-1
sense organs
Signal transduction
Reactive Oxygen Species
Microtubule-Associated Proteins
Food Science
Signal Transduction
Subjects
Details
- ISSN :
- 2042650X
- Volume :
- 10
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Foodfunction
- Accession number :
- edsair.doi.dedup.....0d325c4082a6092fc73219b85de67465