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Spatial distribution of two maternal messengers in Paracentrotus lividus during oogenesis and embryogenesis
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 91(12)
- Publication Year :
- 1994
-
Abstract
- We demonstrated that two mRNAs that are synthesized during the vitellogenic period of oogenesis and that code for cell surface proteins are asymmetrically distributed in the unfertilized egg of Paracentrotus lividus. At fertilization, these RNAs rapidly localize in the cortical zone at the animal pole of the egg. They are then detected in the mesomeres and the macromeres, but not in the micromeres, and thereafter are found in the ectoderm but not in the vegetal plate, mesenchyme cells, or early intestine. They disappear in late gastrula. The proteins synthesized by these mRNAs show the same territorial location during the period examined here, which included the unfertilized egg and the 16-blastomere stage. These conclusions were reached on the basis of in situ hybridization and immunostaining experiments, as well as Northern and Western blot analyses of isolated blastomeres. The possible significance of this asymmetric distribution of these two mRNAs and proteins in the establishment of the animal/vegetal axis is discussed.
- Subjects :
- Multidisciplinary
Polarity in embryogenesis
Embryogenesis
Gene Expression
Membrane Proteins
Ectoderm
In situ hybridization
Blastomere
Biology
biology.organism_classification
Oogenesis
Molecular biology
Paracentrotus lividus
Cell biology
Cell Compartmentation
Gastrulation
medicine.anatomical_structure
Sea Urchins
medicine
Oocytes
Animals
RNA, Messenger
In Situ Hybridization
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 91
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....0d3691f0ec85d4f33b2afce61275a569