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Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2015
-
Abstract
- Parkinson's disease (PD) is a severe neurodegenerative disorder. Although the detailed underlying molecular mechanism remains to be elucidated, the major pathological feature of PD is the loss of dopaminergic (DA) neurons of the substantia nigra. The use of donor stem cells to replace DA neurons may be a key breakthrough in the treatment of PD. In the present study, the growth kinetics of hippocampal neural stem cells (Hip‑NSCs) isolated from postnatal mice and cultured in vitro were observed, specifically the generation of cells expressing DA neuronal markers nuclear receptor related‑1 protein (Nurr1) and tyrosine hydroxylase (TH). It was revealed that Hip‑NSCs differentiated primarily into astrocytes when cultured in serum‑containing medium. However, in low serum conditions, the number of βIII tubulin‑positive neurons increased markedly. The proportion of Nurr1‑positive cells and TH‑positive neurons, significantly increased with increasing duration of directed differentiation of Hip‑NSCs (P=0.0187 and 0.0254, respectively). The results of the present study reveal that Hip‑NSCs may be induced to differentiate in vitro into neurons expressing Nurr1 and TH, known to be critical regulators of DA neuronal fate. Additionally, their expression may be necessary to facilitate neuronal maturation in vitro. These data suggest that Hip‑NSCs may serve as a source of DA neurons for cell therapy in patients diagnosed with PD.
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Tyrosine 3-Monooxygenase
hippocampus
Cellular differentiation
Substantia nigra
Nuclear receptor related-1 protein
Biology
Biochemistry
Cell therapy
03 medical and health sciences
Mice
0302 clinical medicine
Directed differentiation
Neural Stem Cells
Nuclear Receptor Subfamily 4, Group A, Member 2
Genetics
medicine
neurospheres
Animals
Molecular Biology
Cells, Cultured
Tyrosine hydroxylase
Cell Differentiation
Parkinson Disease
Articles
Neural stem cell
Cell biology
Mice, Inbred C57BL
030104 developmental biology
Oncology
nervous system
Animals, Newborn
Gene Expression Regulation
biology.protein
Molecular Medicine
Stem cell
030217 neurology & neurosurgery
primary cell culture
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....0d45dc91409f04a46a504db96ba71aa6