Back to Search
Start Over
Highly Oxidized Peroxisomes Are Selectively Degraded via Autophagy in Arabidopsis[C][W]
- Source :
- The Plant cell, The Plant cell, American Society of Plant Biologists (ASPB), 2013, 25 (12), pp.4967-4983. ⟨10.1105/tpc.113.116947⟩, Plant Cell, Plant Cell, American Society of Plant Biologists, 2013, 25 (12), pp.4967-4983. ⟨10.1105/tpc.113.116947⟩
- Publication Year :
- 2013
- Publisher :
- American Society of Plant Biologists, 2013.
-
Abstract
- The legend for Figure 1B has been corrected; The positioning of peroxisomes in a cell is a regulated process that is closely associated with their functions. Using this feature of the peroxisomal positioning as a criterion, we identified three Arabidopsis thaliana mutants (peroxisome unusual positioning1 [peup1], peup2, and peup4) that contain aggregated peroxisomes. We found that the PEUP1, PEUP2, and PEUP4 were identical to Autophagy-related2 (ATG2), ATG18a, and ATG7, respectively, which are involved in the autophagic system. The number of peroxisomes was increased and the peroxisomal proteins were highly accumulated in the peup1 mutant, suggesting that peroxisome degradation by autophagy (pexophagy) is deficient in the peup1 mutant. These aggregated peroxisomes contained high levels of inactive catalase and were more oxidative than those of the wild type, indicating that peroxisome aggregates comprise damaged peroxisomes. In addition, peroxisome aggregation was induced in wild-type plants by exogenous application of hydrogen peroxide. The cat2 mutant also contained peroxisome aggregates. These findings demonstrate that hydrogen peroxide as a result of catalase inactivation is the inducer of peroxisome aggregation. Furthermore, an autophagosome marker, ATG8, frequently colocalized with peroxisome aggregates, indicating that peroxisomes damaged by hydrogen peroxide are selectively degraded by autophagy in the wild type. Our data provide evidence that autophagy is crucial for quality control mechanisms for peroxisomes in Arabidopsis.
- Subjects :
- 0106 biological sciences
Autophagosome
ATG8
[SDV]Life Sciences [q-bio]
Mutant
Arabidopsis
Plant Science
01 natural sciences
In Brief
03 medical and health sciences
Stress, Physiological
Phagosomes
Autophagy
Peroxisomes
Research Articles
030304 developmental biology
0303 health sciences
biology
Arabidopsis Proteins
Wild type
Hydrogen Peroxide
Cell Biology
Peroxisome
biology.organism_classification
Cell biology
Biochemistry
Catalase
Mutation
biology.protein
Oxidation-Reduction
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 10404651 and 1532298X
- Database :
- OpenAIRE
- Journal :
- The Plant cell, The Plant cell, American Society of Plant Biologists (ASPB), 2013, 25 (12), pp.4967-4983. ⟨10.1105/tpc.113.116947⟩, Plant Cell, Plant Cell, American Society of Plant Biologists, 2013, 25 (12), pp.4967-4983. ⟨10.1105/tpc.113.116947⟩
- Accession number :
- edsair.doi.dedup.....0d65600114fac79de41d1fa076ec2a69