Back to Search
Start Over
Selective and sensitive fluorescence 'turn-on' detection of 4-thiouridine in nucleic acidsviaoxidative amination
- Source :
- Chemical Communications. 55:13096-13099
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- A fluorescence "turn-on" method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed in this work based on the oxidative amination of s4U by fluoresceinamine (FAM-NH2) and periodate (IO4-). It was 125-fold more sensitive for s4U detection than the traditional UV330 absorption method, and showed excellent selectivity to s4U over 2-thiouridine (s2U) analogues and biological thiols.
- Subjects :
- Thiouridine
Oxidative phosphorylation
010402 general chemistry
01 natural sciences
Fluorescence
Catalysis
Turn (biochemistry)
chemistry.chemical_compound
Nucleic Acids
Materials Chemistry
Amination
Molecular Structure
010405 organic chemistry
Chemistry
Metals and Alloys
Periodate
General Chemistry
Combinatorial chemistry
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Nucleic acid
Selectivity
Oxidation-Reduction
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....0d79175e5bca035bca2f4fe43b9a9bc8