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The role of central oxytocin in stress-induced cardioprotection in ischemic-reperfused heart model
- Source :
- Journal of Cardiology. 61(1):79-86
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Background and purpose There is growing evidence that stress contributes to cardiovascular disease and triggers the release of oxytocin. Moreover previous studies confirmed oxytocin mimics the protection associated with ischemic preconditioning. The present study was aimed to assess the possible cardioprotective effects of the centrally released oxytocin in response to stress and intracerebroventricular (i.c.v.) administration of exogenous oxytocin in ischemic-reperfused isolated rat heart. Methods and subjects Rats were divided in two main groups and all of them were subjected to i.c.v. infusion of vehicle or drugs: unstressed rats [control: vehicle, oxytocin (OT; 100 ng/5 μl), atosiban (ATO; 4.3 μg/5 μl) as oxytocin antagonist, ATO + OT] and stressed rats [St: stress, OT + St, ATO + St]. After anesthesia, hearts were isolated and subjected to 30 min regional ischemia and 60 min reperfusion (IR). Acute stress protocol included swimming for 10 min before anesthesia. Myocardial function, infarct size, coronary flow, ventricular arrhythmia, and biochemical parameters such as creatine kinase and lactate dehydrogenase were measured. Ischemia-induced ventricular arrhythmias were counted during the occlusion period. Results The plasma levels of oxytocin and corticosterone were significantly elevated by stress. Unexpectedly hearts of stressed rats showed a marked depression of IR injury compared to control group. I.c.v. infusion of oxytocin mimicked the cardioprotective effects of stress, yet did not elevate plasma oxytocin level. The protective effects of both stress and i.c.v. oxytocin were blocked by i.c.v. oxytocin antagonist. Conclusions These findings suggest that i.c.v. infusion of exogenous oxytocin and centrally released endogenous oxytocin in response to stress could play a role in induction of a preconditioning effect in ischemic-reperfused rat heart via brain receptors.
- Subjects :
- Male
medicine.medical_specialty
Cardiotonic Agents
Ischemia
Ischemia/reperfusion
Myocardial Reperfusion Injury
In Vitro Techniques
Stress
Oxytocin
Oxytocin Antagonist
chemistry.chemical_compound
Corticosterone
Stress, Physiological
Internal medicine
medicine
Animals
Rats, Wistar
Injections, Intraventricular
Cardioprotection
biology
business.industry
Brain
Heart
Atosiban
medicine.disease
Rats
Disease Models, Animal
Endocrinology
chemistry
Receptors, Oxytocin
Ischemic Preconditioning, Myocardial
biology.protein
Ischemic preconditioning
Creatine kinase
business
Cardiology and Cardiovascular Medicine
hormones, hormone substitutes, and hormone antagonists
Stress, Psychological
Receptor
medicine.drug
Subjects
Details
- ISSN :
- 09145087
- Volume :
- 61
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Cardiology
- Accession number :
- edsair.doi.dedup.....0d7b2044f5e4d5e643749f5b5f035194
- Full Text :
- https://doi.org/10.1016/j.jjcc.2012.08.021