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Sapovirus-like particles derived from polyprotein

Authors :
Grant S. Hansman
Tomoichiro Oka
Naokazu Takeda
Source :
Virus Research. 137:261-265
Publication Year :
2008
Publisher :
Elsevier BV, 2008.

Abstract

We expressed full-length sapovirus genome constructs in insect cells and analyzed their products. The capsid protein was cleaved from the ORF1 polyprotein from a native-like genome construct and two full-length genome constructs with mutations in an active polymerase motif, whereas the capsid protein was not cleaved from a full-length genome construct with a mutation in an active protease motif. Our results showed that the sapovirus protease-polymerase precursor protein cleaved the capsid protein from the polyprotein at the putative conserved capsid start. Importantly, the cleaved capsid protein formed empty virus-like particles that were morphologically and antigenically similar to native sapovirus.

Details

ISSN :
01681702
Volume :
137
Database :
OpenAIRE
Journal :
Virus Research
Accession number :
edsair.doi.dedup.....0dc1b98d2589f14692cb95c826f7c36c
Full Text :
https://doi.org/10.1016/j.virusres.2008.07.002