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Impairment of lagging strand synthesis triggers the formation of a RuvABC substrate at replication forks
- Source :
- EMBO Journal, EMBO Journal, EMBO Press, 2001, 20 (3), pp.619-629, HAL
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- The holD gene codes for the psi subunit of the Escherichia coli DNA polymerase III holoenzyme, a component of the gamma complex clamp loader. A holD mutant was isolated for the first time in a screen for mutations that increase the frequency of tandem repeat deletions. In contrast to tandem repeat deletions in wild-type strains, deletion events stimulated by the holD mutation require RecA. They do not require RecF, and hence do not result from the recombinational repair of gaps, arguing against uncoupling of the leading and lagging strand polymerases in the holD mutant. The holD recBC combination of mutations is lethal and holD recBts recCts strains suffer DNA double-strand breaks (DSBs) at restrictive temperature. DSBs require the presence of the Holliday junction-specific enzymes RuvABC and are prevented in the presence of RecBCD. We propose that impairment of replication due to the holD mutation causes the arrest of the entire replisome; consequently, Holliday junctions are formed by replication fork reversal, and unequal crossing over during RecA- and RecBCD-mediated re-incorporation of reversed forks causes the hyper-recombination phenotype.
- Subjects :
- DNA Replication
Replication fork reversal
DNA Repair
DNA repair
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Models, Biological
Article
General Biochemistry, Genetics and Molecular Biology
Substrate Specificity
03 medical and health sciences
RuvABC
Bacterial Proteins
ADN POLYMERASE
Escherichia coli
Holliday junction
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Molecular Biology
ComputingMilieux_MISCELLANEOUS
DNA Polymerase III
DNA Primers
030304 developmental biology
Recombination, Genetic
Genetics
RecBCD
0303 health sciences
Endodeoxyribonucleases
Base Sequence
General Immunology and Microbiology
030306 microbiology
Escherichia coli Proteins
General Neuroscience
DNA Helicases
DNA replication
DNA-Binding Proteins
Rec A Recombinases
Phenotype
Genes, Bacterial
Mutation
bacteria
Replisome
Homologous recombination
Subjects
Details
- ISSN :
- 14602075 and 02614189
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....0dfb2fbb0b0e33463723ae9a9d65575d