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Effects of huperzine A on secretion of nerve growth factor in cultured rat cortical astrocytes and neurite outgrowth in rat PC12 cells
- Source :
- Acta Pharmacologica Sinica. 26:673-678
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- Aim: To study the effects of huperzine A (HupA) on neuritogenic activity and the expression of nerve growth factor (NGF). Methods: After being treated with 10 mumol/L HupA, neurite outgrowth of PC12 cells was observed and counted under phase-contrast microscopy. Mitogenic activity was assayed by [ 3 H]thymidine incorporation. Cell cytotoxicity was evaluated by lactate dehydrogenase (LDH) release. AChE activity, mRNA and protein expression were measured by the Ellman's method, RT-PCR, and Western blot, respectively. NGF mRNA and protein levels were determined by RT-PCR and ELISA assays. Results: Treatment of PC12 cells with 10 mumol/L HupA for 48 h markedly increased the number of neurite-bearing cells, but caused no significant alteration in cell viability or other signs of cytotoxicity. In addition to inhibiting AChE activity, 10 mumol/ L HupA also increased the mRNA and protein levels of this enzyme. In addition, following 2 h exposure of the astrocytes to 10 mumol/L HupA, there was a significant up-regulation of mRNA for NGF and P75 low-affinity NGF receptor. The protein level of NGF was also increased after 24 h treatment with HupA. Conclusion: Our findings demonstrate for the first time that HupA has a direct or indirect neurotrophic activity, which might be beneficial in treatment of neurodegenerative disorders such as Alzheimer disease.
- Subjects :
- Neurite
PC12 Cells
Rats, Sprague-Dawley
chemistry.chemical_compound
Alkaloids
Western blot
Lactate dehydrogenase
Nerve Growth Factor
Neurites
medicine
Animals
Pharmacology (medical)
RNA, Messenger
Cells, Cultured
Huperzine A
Cell Proliferation
Cerebral Cortex
Pharmacology
Plants, Medicinal
biology
medicine.diagnostic_test
Cell growth
Huperzia
General Medicine
Acetylcholinesterase
Molecular biology
Rats
Neuroprotective Agents
Nerve growth factor
chemistry
Astrocytes
biology.protein
Cholinesterase Inhibitors
Sesquiterpenes
medicine.drug
Neurotrophin
Subjects
Details
- ISSN :
- 17457254 and 16714083
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Acta Pharmacologica Sinica
- Accession number :
- edsair.doi.dedup.....0e1b5e98d8c680d92a3bb98281b7b797