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Development of a novel knee contracture mouse model by immobilization using external fixation
- Source :
- Connective Tissue Research. 63:169-182
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Several studies have used animal models to examine knee joint contracture; however, few reports detail the construction process of a knee joint contracture model in a mouse. The use of mouse models is beneficial, as genetically modified mice can be used to investigate the pathogenesis of joint contracture. Compared to others, mouse models are associated with a lower cost to evaluate therapeutic effects. Here, we describe a novel knee contracture mouse model by immobilization using external fixation.The knee joints of mice were immobilized by external fixation using a splint and tape. The passive extension range of motion (ROM), histological and immunohistochemical changes, and expression levels of fibrosis-related genes at 2 and 4 weeks were compared between the immobilized (Im group) and non-immobilized (Non-Im group) groups.The extension ROM at 4 weeks was significantly lower in the Im group than in the Non-Im group (p 0.01). At 2 and 4 weeks, the thickness and area of the joint capsule were significantly greater in the Im group than in the Non-Im group (p 0.01 in all cases). At 2 weeks, the mRNA expression levels of the fibrosis-related genes, except for theThis mouse model may serve as a useful tool to investigate the etiology of joint contracture and establish new treatment methods.
- Subjects :
- musculoskeletal diseases
Contracture
External Fixators
Knee Joint
medicine.medical_treatment
0206 medical engineering
02 engineering and technology
Biochemistry
Immobilization
Mice
03 medical and health sciences
External fixation
Rheumatology
Fracture Fixation
Joint capsule
medicine
Animals
Orthopedics and Sports Medicine
Joint Contracture
Molecular Biology
030304 developmental biology
Orthodontics
0303 health sciences
business.industry
Cell Biology
musculoskeletal system
Fibrosis
020601 biomedical engineering
Knee joint contracture
Disease Models, Animal
medicine.anatomical_structure
medicine.symptom
business
human activities
Joint Capsule
Subjects
Details
- ISSN :
- 16078438 and 03008207
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Connective Tissue Research
- Accession number :
- edsair.doi.dedup.....0e21534427050fca6a1531f3381d7492