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Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and NF-κB signaling pathway
- Source :
- European Journal of Pharmacology. 642:146-153
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- This study aimed to investigate the mechanism underlying the attenuation of LPS-induced lung inflammation by icariin in vivo and in vitro. The anti-inflammatory effects of icariin on LPS-induced acute inflammatory and the molecular mechanism were investigated. Pretreatment with icarrin (20mg/kg) could attenuate acute lung inflammation by inhibiting mRNA expressions of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), metalloproteinase cycloxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in the lung of LPS-treated mice. In addition, icariin suppressed the secretion of TNF-alpha, prostaglandin E2 (PGE(2)) and nitric oxide (NO) as well as NF-kappaB p65 activation. Furthermore, decreased myeloperoxidase (MPO) activity was observed in the lung tissue and LPS-induced cytotoxicity in the RAW 264.7 macrophages cells was also markedly attenuated by icariin. Western blotting analysis and confocal microscopy showed that icariin pretreatment reduced the nucleus transportation and constant level of NF-kappaB p65 in the RAW 264.7 macrophage cells. However, the protective effects of icariin were reversed by a PI3K/Akt inhibitor (wortmannin). Our in vitro and in vivo results suggested that activation of the PI3K/Akt pathway and the inhibition of NF-kappaB were involved in the protective effects of icariin on LPS-induced acute inflammatory responses.
- Subjects :
- Lipopolysaccharides
Male
medicine.medical_specialty
Cell Survival
Inflammation
Pharmacology
Cell Line
Nitric oxide
Wortmannin
Mice
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
Internal medicine
medicine
Animals
RNA, Messenger
Lung
Protein kinase B
PI3K/AKT/mTOR pathway
Peroxidase
Flavonoids
biology
NF-kappa B
Mice, Inbred C57BL
Nitric oxide synthase
Endocrinology
Gene Expression Regulation
chemistry
biology.protein
Tumor necrosis factor alpha
Inflammation Mediators
medicine.symptom
Proto-Oncogene Proteins c-akt
Icariin
Signal Transduction
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 642
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....0e3e5de6c5e5f4e26e5139fc41969f6c