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Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages
- Source :
- Communications Biology, Vol. 4, No 1 (2021) P. 760, Communications Biology, Communications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
- Publication Year :
- 2021
-
Abstract
- PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is implicated in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.<br />Zeeshan et al. used real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches to implicate Plasmodium PP1 in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut. They suggest that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
- Subjects :
- Life Sciences & Biomedicine - Other Topics
Cell biology
Plasmodium
QH301-705.5
Molecular biology
Protozoan Proteins
Medicine (miscellaneous)
Library science
Mitosis
FALCIPARUM
Mosquito Vectors
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
0302 clinical medicine
Political science
Protein Phosphatase 1
parasitic diseases
REVEALS
BINDING
CELL-CYCLE
Biology (General)
PHOSPHORYLATION
Biology
PP1-MEDIATED DEPHOSPHORYLATION
TYPE-1
030304 developmental biology
Cell Proliferation
ddc:616
0303 health sciences
Life Cycle Stages
Science & Technology
KINASES
fungi
3. Good health
Malaria
Multidisciplinary Sciences
Meiosis
MITOSIS
Science & Technology - Other Topics
GROWTH
Core laboratory
General Agricultural and Biological Sciences
Life Sciences & Biomedicine
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Database :
- OpenAIRE
- Journal :
- Communications Biology, Vol. 4, No 1 (2021) P. 760, Communications Biology, Communications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
- Accession number :
- edsair.doi.dedup.....0e427e76bae1ac97c07d891d04c87cbf