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Expanded RNA-binding activities of mammalian Argonaute 2
- Source :
- Nucleic Acids Research
- Publication Year :
- 2009
- Publisher :
- Oxford University Press (OUP), 2009.
-
Abstract
- Mammalian Argonaute 2 (Ago2) protein associates with microRNAs (miRNAs) or small interfering RNAs (siRNAs) forming RNA-induced silencing complexes (RISCs/miRNPs). In the present work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2. Our studies show that recombinant mouse Ago2 binds efficiently to miRNAs forming active RISC. Surprisingly, we find that recombinant mouse Ago2 forms active RISC using pre-miRNAs or long unstructured single stranded RNAs as guides. Furthermore, we demonstrate that, in vivo, endogenous human Ago2 binds directly to pre-miRNAs independently of Dicer, and that Ago2:pre-miRNA complexes are found both in the cytoplasm and in the nucleus of human cells.
- Subjects :
- Ribonuclease III
Small interfering RNA
Recombinant Fusion Proteins
Eukaryotic Initiation Factor-2
Biology
Cell Line
law.invention
Mice
03 medical and health sciences
0302 clinical medicine
law
microRNA
RNA Precursors
Genetics
Animals
Humans
RNA-Induced Silencing Complex
Gene silencing
RNA Processing, Post-Transcriptional
Molecular Biology
030304 developmental biology
Mice, Knockout
0303 health sciences
RNA-Binding Proteins
RNA
Argonaute
Molecular biology
3. Good health
Cell biology
Kinetics
MicroRNAs
Amino Acid Substitution
Ribonucleoproteins
Cytoplasm
Argonaute Proteins
Recombinant DNA
biology.protein
030217 neurology & neurosurgery
Dicer
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....0e4d688a0b66a6f4aafcae57a01e4cda