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The Zebrafish Motility Mutanttwitch onceReveals New Roles for Rapsyn in Synaptic Function
- Source :
- The Journal of Neuroscience. 22:6491-6498
- Publication Year :
- 2002
- Publisher :
- Society for Neuroscience, 2002.
-
Abstract
- Upon touch, twitch once zebrafish respond with one or two swimming strokes instead of typical full-blown escapes. This use-dependent fatigue is shown to be a consequence of a mutation in the tetratricopeptide domain of muscle rapsyn, inhibiting formation of subsynaptic acetylcholine receptor clusters. Physiological analysis indicates that reduced synaptic strength, attributable to loss of receptors, is augmented by a potent postsynaptic depression not seen at normal neuromuscular junctions. The synergism between these two physiological processes is causal to the use-dependent muscle fatigue. These findings offer insights into the physiological basis of human myasthenic syndrome and reveal the first demonstration of a role for rapsyn in regulating synaptic function.
- Subjects :
- Repetitive Sequences, Amino Acid
Patch-Clamp Techniques
Recombinant Fusion Proteins
Green Fluorescent Proteins
Muscle Proteins
Motility
In Vitro Techniques
Motor Endplate
Membrane Potentials
Animals, Genetically Modified
Escape Reaction
Postsynaptic potential
Animals
Receptors, Cholinergic
Patch clamp
ARTICLE
Muscle, Skeletal
Receptor
Zebrafish
Acetylcholine receptor
Behavior, Animal
Muscle fatigue
biology
General Neuroscience
Homozygote
Receptor Aggregation
biology.organism_classification
Electric Stimulation
Luminescent Proteins
Tetratricopeptide
Muscle Fatigue
Synapses
Neuroscience
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....0e587bb0de2982cf2e28ab910e9e35e3