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Effect of high-fat diet on the expression of proteins in muscle, adipose tissues, and liver of C57BL/6 mice
- Source :
- Proteomics, Vol. 4, No 8 (2004) pp. 2270-82
- Publication Year :
- 2004
-
Abstract
- In the present study, the effect of a high fat diet on the expression of proteins in insulin target tissues was analyzed using a proteomic approach. Gastrocnemius muscle, white and brown adipose tissue, and liver were taken from C57BL/6 mice either fed on a high-fat or a chow diet. Expression levels of approximately 10 000 polypeptides for all the four tissues were assessed by two-dimensional gel electrophoresis (2-DE). Computer-assisted image analysis allowed the detection of 50 significantly (p < 0.05) differentially expressed proteins between obese and lean mice. Interestingly, more than half of these proteins were detected in the brown adipose tissue. The differentially expressed proteins were identified by tandem mass spectrometry. Several stress and redox proteins were modulated in response to the high-fat diet. A key glycolytic enzyme was found to be downregulated in adipose tissues and muscle, suggesting that at elevated plasma fatty acid concentrations, fatty acids compete with glucose as an oxidative fuel source. Furthermore, in brown adipose tissue there were significant changes in mitochondrial enzymes involved in the Krebs tricarboxylic acid (TCA) cycle and in the respiratory chain in response to the high-fat diet. The brown adipose tissue is an energy-dissipating tissue. Our data suggest that the high-fat diet treated mice were increasing energy expenditure to defend against weight gain.
- Subjects :
- Adipose Tissue/metabolism
Liver/metabolism
medicine.medical_specialty
Adipose tissue macrophages
Respiratory chain
Adipose tissue
White adipose tissue
Biology
Biochemistry
Models, Biological
Mass Spectrometry
Mice
Insulin resistance
Internal medicine
Obesity/metabolism
Brown adipose tissue
medicine
Animals
Humans
Electrophoresis, Gel, Two-Dimensional
Obesity
ddc:576
Muscle, Skeletal
Molecular Biology
Dietary Fats/metabolism
chemistry.chemical_classification
Muscle, Skeletal/metabolism
Fatty acid
Glucose Tolerance Test
medicine.disease
Dietary Fats
Mice, Inbred C57BL
medicine.anatomical_structure
Endocrinology
chemistry
Adipose Tissue
Liver
Biological Markers
medicine.symptom
Insulin Resistance
Insulin Resistance/physiology
Weight gain
Biomarkers
Subjects
Details
- ISSN :
- 16159853
- Volume :
- 4
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Proteomics
- Accession number :
- edsair.doi.dedup.....0e671c71c3031fffff99c1a68f505a36