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An alternative approach to produce versatile retinal organoids with accelerated ganglion cell development
- Source :
- Scientific Reports, 11(1):1101. Nature Publishing Group, Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021), Scientific Reports, Scientific Reports, 11(1). Nature Publishing Group, Scientific reports, 11(1):1101. Nature Publishing Group
- Publication Year :
- 2021
-
Abstract
- Genetically complex ocular neuropathies, such as glaucoma, are a major cause of visual impairment worldwide. There is a growing need to generate suitable human representative in vitro and in vivo models, as there is no effective treatment available once damage has occured. Retinal organoids are increasingly being used for experimental gene therapy, stem cell replacement therapy and small molecule therapy. There are multiple protocols for the development of retinal organoids available, however, one potential drawback of the current methods is that the organoids can take between 6 weeks and 12 months on average to develop and mature, depending on the specific cell type wanted. Here, we describe and characterise a protocol focused on the generation of retinal ganglion cells within an accelerated four week timeframe without any external small molecules or growth factors. Subsequent long term cultures yield fully differentiated organoids displaying all major retinal cell types. RPE, Horizontal, Amacrine and Photoreceptors cells were generated using external factors to maintain lamination.
- Subjects :
- Retinal Ganglion Cells
Genetic enhancement
Science
Stem-cell differentiation
Developmental neurogenesis
Biology
Retinal ganglion
Retina
Article
Cell Line
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
medicine
Organoid
Humans
Photoreceptor Cells
030304 developmental biology
0303 health sciences
Multidisciplinary
Cell growth
Cell Differentiation
Retinal
Ganglion
Cell biology
Organoids
medicine.anatomical_structure
chemistry
Medicine
Ganglia
Gene expression
Stem cell
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, 11(1):1101. Nature Publishing Group, Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021), Scientific Reports, Scientific Reports, 11(1). Nature Publishing Group, Scientific reports, 11(1):1101. Nature Publishing Group
- Accession number :
- edsair.doi.dedup.....0e800050d517e14bc2fa7480a41d2406