Additional file 1 of Fibrotic alterations in human annulus fibrosus correlate with progression of intervertebral disc herniation

Additional file 1: Supplementary Materials and Methods. Supplementary Table S1: Donor information regarding gender, age, Pfirrmann scale and condition. Supplementary Figure S1: Image analysis workflow. Schematization of the image analysis process, for both ECM and cellular markers. Supplementary Figure S2: Human AF mosaic representation with histological staining Alcian Blue/Picro-Sirius Red. Distinct hernia containment levels are presented, as follows (a) AIS, (b) Protused, (c) Contained and (d) Uncontained. Rectangles indicate the area where staining analysis was further conducted. Scale bar: 500 ��m. Supplementary Figure S3: Example representation of negative IHC controls for each staining. (a) Collagen I; (b) Collagen II; (c) Fibronectin, scale bar: 100 ��m; (d) ��-SMA; (e) MMP12, scale bar: 50 ��m.