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Reducing heterophilic antibody interference in immunoassays using single-chain antibodies
- Source :
- Analytical Biochemistry. 419:333-335
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Sandwich enzyme-linked immunosorbent assay (ELISA) microarrays can simultaneously quantify the levels of multiple diagnostic targets in a biological sample. However, as with traditional ELISA diagnostics, endogenous antibodies in patient sera can cause interference. We demonstrate here that reducing the diagnostic capture antibody to its minimal functional unit (i.e., a single-chain antibody fragment [scFv]) is an effective strategy for reducing assay interference. Our finding illustrates a source of error introduced by the reliance on immunoglobulin-based capture reagents in sandwich immunoassays with human serum samples. We demonstrate that scFvs can be used in such assays to improve reliability by reducing heterophilic antibody interference, thereby improving biomarker analysis and validation.
- Subjects :
- Biophysics
Antibodies, Heterophile
Biochemistry
Immunoglobulin G
Mice
medicine
Animals
Humans
Hook effect
Biomarker Analysis
Molecular Biology
Immunoassay
medicine.diagnostic_test
biology
Chemistry
Cell Biology
Virology
Molecular biology
biology.protein
Protein microarray
Antibody
DNA microarray
Single-Chain Antibodies
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 419
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....0ea575b8534cc3b649106932196f2595