Supplementary Experimental Materials and Methods, Figure S1-4, Table S1-3 from 2-Hydroxylation of Fatty Acids Represses Colorectal Tumorigenesis and Metastasis via the YAP Transcriptional Axis

Supplementary Experimental Materials and Methods. Supplementary Figure S1. Expression of FA2H in human colorectal cancer cells. Supplementary Figure S2. siRNA-mediated FA2H knockdown promotes HCT116 cell proliferation and migration. Supplementary Figure S3. Regulation of Gli1 and phosphorylation of ERK1/2, p38, and JNK by FA2H. Supplementary Figure S4. FA2H promotes AMPK phosphorylation in CRC cells. Supplementary Table S1. Association between FA2H and clinic-pathological factors in 141 patients with colorectal cancer. Supplementary Table S2. Antibody information. Supplementary Table S3. Primers used for qRT-PCR analysis.