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The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors against Methicillin-ResistantStaphylococcus aureus

Authors :
Dong-Yeul Kwon
Ok-Hwa Kang
Kuang-Shim Lee
Myong-Soo Chong
Dong-Sung Lee
Jang-Gi Choi
Sung-Bae Kim
Dae-Ki Joung
Su-Hyun Mun
Youn-Chul Kim
Dong-Won Shin
Ryong Gong
Source :
Evidence-based Complementary and Alternative Medicine : eCAM, Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014)
Publication Year :
2014
Publisher :
Hindawi Limited, 2014.

Abstract

Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome ofBelamacanda chinensis(L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistantStaphylococcus aureus(MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived fromS. aureus. Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes inS. aureusmorphology. The MIC values of TTR against all the tested strains were 125 μg/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN3with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μg/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μg/mL directly bind to and inhibit TTR at 62.5 μg/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains.

Details

ISSN :
17414288 and 1741427X
Volume :
2014
Database :
OpenAIRE
Journal :
Evidence-Based Complementary and Alternative Medicine
Accession number :
edsair.doi.dedup.....0f06637d3b965e432cd6ea98b882d07b
Full Text :
https://doi.org/10.1155/2014/716509