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The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors against Methicillin-ResistantStaphylococcus aureus
- Source :
- Evidence-based Complementary and Alternative Medicine : eCAM, Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Hindawi Limited, 2014.
-
Abstract
- Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome ofBelamacanda chinensis(L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistantStaphylococcus aureus(MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived fromS. aureus. Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes inS. aureusmorphology. The MIC values of TTR against all the tested strains were 125 μg/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN3with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μg/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μg/mL directly bind to and inhibit TTR at 62.5 μg/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains.
- Subjects :
- Tectorigenin
endocrine system
Article Subject
biology
Membrane permeability
business.industry
ATPase
nutritional and metabolic diseases
lcsh:Other systems of medicine
lcsh:RZ201-999
medicine.disease_cause
Antimicrobial
Microbiology
Multiple drug resistance
chemistry.chemical_compound
Complementary and alternative medicine
chemistry
Staphylococcus aureus
medicine
biology.protein
Peptidoglycan
business
Antibacterial activity
Research Article
Subjects
Details
- ISSN :
- 17414288 and 1741427X
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....0f06637d3b965e432cd6ea98b882d07b
- Full Text :
- https://doi.org/10.1155/2014/716509